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Synthetic Fire Ant Venom Transcriptosome Analysis

ABSTRACT: Human U937 cells were maintained in RPMI 1640 media. Solenopsin B added at a concentration equivalent to the IC50, 13 micro Molar. Triplicate cultures were prepared at two separate time points, 1 and 6 hours. Keywords: Transcriptosome analysis employing DNA/cDNA glass microarray.

ORGANISM(S): Homo sapiens

PROVIDER: GSE13102 | GEO | 2009/10/01

SECONDARY ACCESSION(S): PRJNA109931

REPOSITORIES: GEO

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Synthetic Fire Ant Venom Transcriptosome Analysis

Project description:Human U937 cells were maintained in RPMI 1640 media. Solenopsin B added at a concentration equivalent to the IC50, 13 micro Molar. Triplicate cultures were prepared at two separate time points, 1 and 6 hours. Keywords: Transcriptosome analysis employing DNA/cDNA glass microarray. Two-condition experiment, U937 cell and Sol B treated U937 cells at two time points: 1 and 6 hours post exposure. Biological replicates: 3, independently grown and harvested. One replicate per array.

2010-05-22 | E-GEOD-13102 | biostudies-arrayexpress

Fire Ant Venom Alkaloids Inhibit Biofilm Formation.

Project description:Biofilm formation on exposed surfaces is a serious issue for the food industry and medical health facilities. There are many proposed strategies to delay, reduce, or even eliminate biofilm formation on surfaces. The present study focuses on the applicability of fire ant venom alkaloids (aka 'solenopsins', from Solenopsis invicta) tested on polystyrene and stainless steel surfaces relative to the adhesion and biofilm-formation by the bacterium Pseudomonas fluorescens. Conditioning with solenopsins demonstrates significant reduction of bacterial adhesion. Inhibition rates were 62.7% on polystyrene and 59.0% on stainless steel surfaces. In addition, solenopsins drastically reduced cell populations already growing on conditioned surfaces. Contrary to assumptions by previous authors, solenopsins tested negative for amphipathic properties, thus understanding the mechanisms behind the observed effects still relies on further investigation.

| S-EPMC6669452 | biostudies-literature

The allergic response mediated by fire ant venom proteins.

Project description:Fire ants are widely studied, invasive and venomous arthropod pests. There is significant biomedical interest in immunotherapy against fire ant stings. However, mainly due to practical reasons, the physiological effects of envenomation has remained poorly characterized. The present study takes advantage of a recently-described venom protein extract to delineate the immunological pathways underlying the allergic reaction to fire ant venom toxins. Mice were injected with controlled doses of venom protein extract. Following sensitization and a second exposure, a marked footpad swelling was observed. Based on eosinophil recruitment and production of Th2 cytokines, we hereby establish that fire ant proteins per se can lead to an allergic response, which casts a new light into the mechanism of action of these toxins.

| S-EPMC6158280 | biostudies-literature

RNA-seq analysis of the effect of BSA and NaOH added to standard culturing medium on THP-1 macrophages

Project description:The data is supplementary to the RNA-seq analysis of LPS and palmitate stimulation of THP-1 macrophages (E-MTAB-6064), where palmitate for the cell treatment was dissolved in sodium hydroxide and coupled with BSA at a molar ratio 7.5:1. Here we stimulated THP-1 macrophages with the corresponding concentration of BSA (4%) and NaOH (0.4 mM) in the presence of 10 nM phorbol 12-myristate 13-acetate (PMA) for 24 hours added to the standard RPMI 1640 medium with 10% fetal bovine serum (FBS) to estimate the effects and compared them with unstimulated THP-1 macrophages cultured in RPMI 1640 medium with 10% FBS and 10nM PMA.

2019-05-17 | E-MTAB-6439 | biostudies-arrayexpress

U937 cells: differentiating vs. undifferentiated

Project description:Gene-expression changes in U937 cells undergoing monocytic differentiation with TPA treatment Keywords: differentiation treatment The promonocytic U937 cell line was maintained at 37C in RPMI 1640 medium (Mediatech) supplemented with 10% fetalclone (HyClone) under a 5% CO2, 95% air atmosphere. U937 cells were differentiated by the addition to the growth medium 100 nM 12-O-tetradeconoyl-phorbol 13-acetate (TPA) (Sigma).Three condition experiment, treated with TPA for two different time points (27 and 96 hrs) vs. untreated. Biological replicates: independently grown, treated and harvested

2010-06-20 | E-GEOD-10560 | biostudies-arrayexpress

Fire ant worker brain transcriptomes

Project description:Transcriptomes of dissected brains from workers from polygyne fire ants were analyzed. Four replicates were obtained.

2020-07-18 | GSE117432 | GEO

To investigate whether recover DNMT3A expression permitted adult beta cell of βRapKO establishing proper transcriptome

Project description:Murine islets were isolated from 8-week-old WT and βRapKO mice and initially recovered in 1640 RPMI supplemented with 10% serum, 11.1mM glucose overnight. Then islets were transfected with Lenti-GFP or Lenti-Dnmt3a for 24 hours, following cultured in 1640 RPMI medium for 48 hours.Islets were harvested and stored in -80°C for preparation for RNA sequencing.

2019-06-01 | GSE130910 | GEO

Microarray Analysis of Genes Regulated by Macrophage-Conditioned Media Treatment of Human Pre-Adipocytes

Project description:A major development in the study of obesity is the recognition that the condition is characterised by chronic mild inflammation. Within adipose tissue, this involves the infiltration of macrophages, as well as the direct inflammatory response of the adipocytes and pre-adipocytes. This study has used Agilent whole-genome microarrays to examine the effects of macrophage-conditioned medium on the global inflammatory response of human pre-adipocytes. Human pre-adipocytes (SGBS cells) were treated with macrophage (U937 cells) conditioned medium for 24 h. Control pre-adipocytes were treated with unconditioned medium (control) or RPMI-1640 media alone to control for differences in media used to culture the U937 cells. There were 5 biological replicates per group.

2011-12-21 | E-GEOD-27503 | biostudies-arrayexpress

LNCaP prostate cancer cell line spheres (S) vs. adherent (A)

Project description:Transcriptional profile of LNCaP spheres in SCM-1% KO (stem-like cells) vs adherent cultures in RPMI-1640-10% FBS (differentiated-like cells)

2009-12-31 | GSE19704 | GEO

Major venom proteins of the fire ant Solenopsis invicta: insights into possible pheromone-binding function from mass spectrometric analysis.

Project description:Proteins in the venom of the fire ant Solenopsis invicta have been suggested to function in pheromone binding. Venom from queens and workers contains different isoforms of these proteins, consistent with the differing pheromones they secrete, but questions remain about the venom protein composition and glandular source. We found that the queen venom contains a previously uncharacterized pheromone-binding protein paralogue known as Sol i 2X1. Using imaging mass spectrometry, we located the main venom proteins in the poison sac, implying that pheromones might have to compete with venom alkaloids for binding. Using the known structure of the worker venom protein Sol i 2w, we generated three-dimensional homology models of the worker venom protein Sol i 4.02, and of the two main venom proteins in queens and female alates, Sol i 2q and Sol i 2X1. Surprisingly, the models show that the proteins have relatively small internal hydrophobic binding pockets that are blocked by about 10 amino acids of the C-terminal region. For these proteins to function as carriers of hydrophobic ligands, a conformational change would be required to displace the C-terminal region, somewhat like the mechanism known to occur in the silk moth pheromone-binding protein.

| S-EPMC6188847 | biostudies-literature

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