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Synthetic Fire Ant Venom Transcriptosome Analysis

ABSTRACT: Human U937 cells were maintained in RPMI 1640 media. Solenopsin B added at a concentration equivalent to the IC50, 13 micro Molar. Triplicate cultures were prepared at two separate time points, 1 and 6 hours. Keywords: Transcriptosome analysis employing DNA/cDNA glass microarray.

ORGANISM(S): Homo sapiens

PROVIDER: GSE13102 | GEO | 2009/10/01

SECONDARY ACCESSION(S): PRJNA109931

REPOSITORIES: GEO

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Synthetic Fire Ant Venom Transcriptosome Analysis

Project description:Human U937 cells were maintained in RPMI 1640 media. Solenopsin B added at a concentration equivalent to the IC50, 13 micro Molar. Triplicate cultures were prepared at two separate time points, 1 and 6 hours. Keywords: Transcriptosome analysis employing DNA/cDNA glass microarray. Two-condition experiment, U937 cell and Sol B treated U937 cells at two time points: 1 and 6 hours post exposure. Biological replicates: 3, independently grown and harvested. One replicate per array.

2010-05-22 | E-GEOD-13102 | biostudies-arrayexpress

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Project description:2,6-dialkylpiperideines found in the venom of Solenopsis (Hymenoptera, Formicidae) fire ants are a range of compounds possessing various biological activities. A series of racemic 2-methyl-6-alkyl-Δ1,6-piperideines were synthesized for chemical confirmation of the natural products found in fire ant venom, and the evaluation of their biological activity. Synthetic Δ1,6-piperideines and the natural compounds in the cis-alkaloid fraction of Solenopsis invicta had identical mass spectra and retention times. Their insecticidal activities against the third-instar larvae of cotton bollworm (Helicoverpa armigera) were evaluated by using injection and topical application methods. All three compounds exhibited no lethal effect at concentrations of 0.05-0.4 mol/L by topical treatment, but moderate lethal effect at 0.4 mol/L through injection treatment. Compound 6a showed significantly higher activity than the natural insecticide nicotine. The differences in activity among compounds 6b, 6c and nicotine were not significant. The elongation of the carbon chain at the 6-position of the piperideine ring appears to decrease insecticidal activity.

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