Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from Jurkat cell line infected with pMIN-ERG+85 vector

ABSTRACT: Jurkat cells infected with pMIN-ERG+85 express different tagBFP levels. Using flow cytometry sorting, highest and lowest tagBFP cells (10%) were sorted. High tagBFP cells are functionaly have more leukemia stem cell proprties in comparision to low tagBFP cells. Utilizing sorted cells to identifiy differential transcriptomics between two subpopulations We used microarrays to detail the global programme of gene expression underlying cellularisation and identified distinct classes of up/down-regulated genes that determine High tagBFP cells vs Low tagBFP

ORGANISM(S): Homo sapiens

PROVIDER: GSE131079 | GEO | 2019/05/14

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

An autophagy CRISPR Screen in Jurkat cells

Project description:To identify genes regulating autophagy in human T-cells (Jurkat cell line) a CRISPR screen was conducted. Populations with low autophagic flux was sorted and the contained sgRNAs analysed by next-generation sequencing. Bioinformatic analysis compared the input of the screen versus the 'autophagy low' population.

2021-01-13 | GSE147488 | GEO

Jurkat T cells

Project description:Jurkat T cells

| PRJNA958225 | ENA

Expression data from Jurkat cell line infected with pMIN-ERG+85 vector

Project description:Expression data from Jurkat cell line infected with pMIN-ERG+85 vector

| PRJNA542620 | ENA

Identification of LEDGF-responsive genes in Jurkat cells

Project description:The LEDGF transcript from the PSIP1 gene was knocked down in Jurkat cells using RNAi technology. The resulting Jurkat-derived cell line (Jurkat-siJK2) was compared to a control cell line (wild type Jurkat) using microarray analysis. Genes identified as being modulated by LEDGF were preferential targets of HIV integration. Keywords: effects of gene knockdown

2007-04-13 | GSE7508 | GEO

Nef transfection of Jurkat T cells

Project description:Jurkat T cell line was transfected with SIV Nef controlled by an unducible promoter system. Nef was induced by addition of 10 micromolar pronasterone A and gene expression values were determined after 24 hrs. Control Jurkat cells were untransfected and treated with 10 micromolar pronasterone A and gene expressio values were determined after 24 hrs. Keywords: SIV Nef, Jurkat, pronasterone A

2006-07-25 | GSE4785 | GEO

Short-term hypergravity effects on Jurkat T cells

Project description:Jurkat T cells have been exposed to 9g hypergravity in a custom-built pipette centrifuge for different times (GBF2021). Additionally, Jurkat T cells have been exposed to 300g for 5 minutes in a standard benchtop centrifuge, and waited for different times until adding lysis buffer. For comparison, Jurkat T cells have been exposed to 5 minutes of 42°C.

2023-02-07 | GSE221579 | GEO

role of miR-146a on Jurkat T cells

Project description:screenning the differentially expressed genes of Jurkat-FF3, Jurkat-miR146a, Jurkat-miR146a-sponge cell lines. To investigate the role of miR-146a on Jurkat T cells, we screened the differentially expressed genes of Jurkat-FF3 (as control), Jurkat-miR146a (as miR-146a overexpression cell line), Jurkat-miR146a-sponge (as miR-146a knock down cell line).

2010-04-03 | E-GEOD-21132 | biostudies-arrayexpress

Gene expression in Jurkat T cell lines

Project description:Jurkat cell lines were generated to stably express CD46 protein expressing either cytoplasmic tail 1 or 2 (BC1 or BC2). The transcription profile of these unactivated stable lines were compared with unactivated Jurkat cells.

2019-01-29 | GSE122499 | GEO

role of miR-146a on Jurkat T cells

Project description:screenning the differentially expressed genes of Jurkat-FF3, Jurkat-miR146a, Jurkat-miR146a-sponge cell lines.

2010-04-03 | GSE21132 | GEO

Kinome analysis of CD151 overexpressing Jurkat T cells

Project description:The effect of CD151 expression onto the kinome of Jurkat T cells was assessed using kinome analysis. CD151 was expressed in Jurkat T cells by retroviral transduction based on a pMSCV vector. Entrez Gene: 977 UniProtKB: P48509 Jurkat T cells were transduced with the MSCV-CD151 vector and successfully transduced cells were selected using puromycin. For the kinome array experiments 3 independent samples of Jurkat cells and three independent samples of J-CD151 cells were collected. To minimize unspecific background signals, lysates from Jurkat and J-CD151 T cells harvested at different growth stages, which were then pooled to provide one sample prior to loading on the Kinexus antibody microarrays.

2017-09-30 | E-MTAB-5978 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data