Transcriptomics

Dataset Information

0

Gene expression profiles between miRNA Negative control-treated and miR-124a-3p mimic treated-teratomas


ABSTRACT: The role of microRNAs (miRNAs) during mouse early development, especially in endoderm germ layer formation, is largely unknown. Here, using miRNA profiling, we discovered that miR-124a negatively regulates endoderm lineage commitment in mouse embryonic stem cells (mESCs). We showed that miR-124a inhibits endoderm differentiation in vitro through targeting the 3’ untranslated region (UTR) of Sox17 and Gata6, revealing the existence of an interplay between miR-124a and Sox17/Gata6 transcription factors in hepato-specific gene regulation. Besides, we proposed an in vivo system that utilizes teratoma to evaluate the functional role of miRNA in lineage specification. We demonstrated that ectopic expression of miR-124a in teratomas suppressed endoderm and mesoderm lineage differentiation while augmented the differentiation of ectoderm lineage. Collectively, our findings suggested that miR-124a plays a significant role in mESCs lineage commitment.

ORGANISM(S): Mus musculus

PROVIDER: GSE131346 | GEO | 2019/12/31

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2019-12-31 | GSE131337 | GEO
2023-07-09 | GSE213661 | GEO
2012-06-04 | E-GEOD-38477 | biostudies-arrayexpress
2023-07-20 | PXD039633 | Pride
2015-07-30 | E-GEOD-71528 | biostudies-arrayexpress
2012-06-05 | GSE38477 | GEO
2015-07-30 | GSE71528 | GEO
2020-07-26 | GSE143499 | GEO
2021-07-01 | MODEL2105030001 | BioModels
2024-09-02 | BIOMD0000000209 | BioModels