Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide maps of Bud27 binding to the chromatin [ChIP-seq]

ABSTRACT: The goal of this study is to analyse genome-wide the binding profile of Bud27 prefoldin-like to the genes or other genomic regions in order to know which genes could be transcriptionally regulated by this protein and to elucidate the contribution of Bud27 to the transcription. Average normalised ChIP-seq signal for Bud27 prefoldin-like binding in a S.cerevisiae wild-type strain.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE131388 | GEO | 2020/06/22

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Genome-wide binding of Rpb1 (the largest subunit of the RNA polymerase II) to the chromatin state in bud27∆ and wild-type mutant strains (ChIP-seq)

Project description:Comparison of the average normalised ChIP-seq signal over protein-coding genes in bud27∆ mutants strain and wild-type.

2020-06-22 | GSE134534 | GEO

RSC and Bud27 influences chromatin organization and alternative polyadenylation

Project description:The association of the RSC remodeler complex with the chromatin depends on the prefoldin-like Bud27 and determines the nucleosome positioning and polyadenylation sites usage in Saccharomyces cerevisiae

2024-03-01 | GSE226720 | GEO

ChIP-seq data of prefoldin subunit PFDN5 in HCT116 human colon carcinoma cells

Project description:Prefoldin is a heterohexameric complex conserved from archaea to humans that plays a cochaperone role during the co-translational folding of actin and tubulin monomers. Additional functions of prefoldin in the cell nucleus have been described, including a positive contribution to transcription elongation and chromatin dynamics in yeast. Here we show that prefoldin perturbations provoked transcriptional alterations across the genome of human cells. We detected genome-wide prefoldin binding to transcribed genes and found that this binding negatively correlate with the impact of prefoldin depletion on gene expression. Moreover, lack of prefoldin caused significant global decrease in the levels of Ser2 and Ser5 phosphorylation of the RNA polymerase II carboxy-terminal domain (CTD). Altogether the reported results indicate that human prefoldin is able to act locally on the genome to modulate gene expression by influencing phosphorylation of elongating RNA polymerase II, and thereby regulating co-transcriptional splicing.

2021-05-04 | GSE171466 | GEO

Context dependent function of transcriptional regulator Rap1 in gene silencing and activation [ChIP-seq III]

Project description:As part of a study on the mechanism of Sir-based silencing at HML in S.cerevisiae, and the contribution of Rap1 to transcription of the locus, we performed ChIP-seq of multiple components of RNA Pol II machinery in different genetic backgrounds including: sir4∆, SIR+, and with or without a mutation that abbrogated Rap1 binding at the alpha promoter.

2023-05-09 | GSE227760 | GEO

Comparative genomics reveals Chd1 as a determinant of nucleosome spacing in vivo

Project description:We generated S.cerevisiae strains in which endogenous copies of candidate nucleosome spacing factors were replaced with the K.lactis copies. With this candidate approach, we found that K.lactis Chd1 directed longer nucleosome repeat length in S.cerevisiae. Generating chimeric proteins revealed that the strongest contribution to this differential spacing lies in the undercharacterised N-terminus of Chd1.

2015-03-18 | GSE66979 | GEO

DNA methylation screening of small bowel adenocarcinoma

Project description:Illumina Infinium 450k screening of normal and tumour tissue in a retrospective 28 sample cohort of small bowel adenocarcinoma patients. Average signal and beta values normalised to internal Illumina controls.

2015-09-03 | GSE61467 | GEO

Genome-wide maps of Bud27 binding to the chromatin [ChIP-seq]

Project description:Genome-wide maps of Bud27 binding to the chromatin [ChIP-seq]

| PRJNA543472 | ENA

WGD paralog evolution

Project description:Studying the evolution of binding preferences for 30 paralog TF pairs in S.cerevisiae

2022-01-21 | GSE179430 | GEO

Genome-wide maps of ARID1A binding genes in H9 human embryonic stem cells

Project description:We reported loss of ARID1A promoted neurogenesis and inhibited cardiogenesis. Here we used specific ARID1A antibody to pull down ARID1A binding genomic DNA in human embryonic stem cells, which let us know the potential genes regulated by ARID1A during neurogenesis and inhibited cardiogenesis. IgG was used as the control.

2023-06-11 | GSE152324 | GEO

Genome-wide maps of ARID1A binding genes in H9 human embryonic stem cells.

2020-06-19 | GSE139260 | GEO