Effects of Single and Combined Low Concentrations of Neuroactive Drugs on Daphnia magna Reproduction and Transcriptomic Responses

ABSTRACT: Transcriptomic profiling of Daphnia magna samples exposed to carbamazepine (CBZ), diazepan (DZP), propranolol (PR) and to an equitoxic mixture of them (M). Assessing the risks of emerging contaminants such as neuro-active pharmaceuticals in the environment requires an understanding of their joint effects at low concentrations across species. Here, we assessed reproductive and transcriptional effect of propranolol, diazepam and carbamazepine on the crustacean Daphnia magna at environmentally relevant concentrations. The three compounds enhanced reproductive parameters in adults, and induced specific transcriptome changes in pre-adolescent individuals. Comparing results from single exposures and of ternary equi-effective mixtures showed additive action for both effects. Transcriptomic analyses identified 3248 genes affected by at least one of the treatment, which were grouped into four clusters. Two clusters included 1897 gene transcripts in total, that behaved similarly (appearing either over- or underrepresented relative to control) in single and mixture treatments. The third and fourth clusters grouped genes differently transcribed upon exposure to diazepam and propranolol, respectively. Functional transcriptomics indicated that the four clusters shared major de-regulated signalling pathways implicated on energy, growth, reproduction, and neurologically- related processes, which may be responsible for the observed reproductive effects. Our study, thus, showed additive effects at the transcriptional and physiological level and is providing a novel approach to the analysis of environmental relevant mixtures of neuro-active compounds

ORGANISM(S): Daphnia magna

PROVIDER: GSE131587 | GEO | 2020/05/20

REPOSITORIES: GEO