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Single cell RNA sequencing of colorectal cancer with diverse tissue dissociation protocols or fresh vs. frozen cell preparations.

ABSTRACT: To evaluate 3 different tissue dissociation protocols or fresh vs. frozen cell preparations, we performed single-cell RNA sequencing on cancer or distant normal tissue dissociates from 2 colorectal cancer patients. Total 18,409 cells from 10 sample preparations were analyzed (5 primary colorectal cancer and 5 matched normal mucosa). The results suggest highly consistent cellular proportions were recovered with different sample preparation methods.

ORGANISM(S): Homo sapiens

PROVIDER: GSE132257 | GEO | 2020/04/04

REPOSITORIES: GEO

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Fresh vs. frozen cell preparations of colorectal cancer single-cell RNA sequencing

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