Transcriptomics

Dataset Information

0

Fc receptor-like 6 (FCRL6) defines pre-BCR dependent and independent pathways of natural antibody selection


ABSTRACT: B-1a cells produce ‘natural’ antibodies (Abs) to neutralize pathogens and clear neo self-antigens, but the fundamental selection mechanisms that shape their polyreactive repertoires are poorly understood. Here, we identified a B cell progenitor subset defined by Fc receptor-like 6 (FCRL6) expression, harboring innate-like defense, migration, and differentiation properties conducive for natural Ab generation Compared to FCRL6- pro B cells, the repressed mitotic, DNA damage repair, and signaling activity of FCRL6+ progenitors, yielded VH repertoires with biased distal Ighv segment accessibility, constrained diversity, and hydrophobic and charged CDR-H3 sequences. Beyond nascent autoreactivity, VH11 productivity, which predominates phosphatidylcholine-specific B-1a B cell receptors (BCRs), was higher for FCRL6+ cells as was pre-BCR formation, which was required for Myc induction and VH11, but not VH12, B-1a development. Thus, FCRL6 revealed unexpected heterogeneity in the developmental origins, regulation, and selection of natural Abs at the pre-BCR checkpoint with implications for autoimmunity and lymphoproliferative disorders.

ORGANISM(S): Mus musculus

PROVIDER: GSE132438 | GEO | 2020/02/26

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2020-02-21 | GSE140795 | GEO
2023-12-17 | GSE250175 | GEO
2023-12-11 | PXD047089 | Pride
2018-01-15 | GSE104111 | GEO
2021-12-16 | GSE164147 | GEO
2019-08-27 | GSE135603 | GEO
2018-06-22 | E-MTAB-6386 | biostudies-arrayexpress
2024-04-15 | GSE238082 | GEO
2024-05-15 | GSE267440 | GEO
2024-12-05 | GSE277475 | GEO