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Photodamage repair pathways contribute to the accurate maintenance of DNA methylome landscape upon UV exposure

ABSTRACT: We report the methylation level of photodamage repair deficient plants 24h upon UV-C exposure. We determined the methlome changes genome wide and at photodamaged sites using NGS approaches.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE132750 | GEO | 2019/11/01

REPOSITORIES: GEO

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Photodamage repair pathways contribute to the accurate maintenance of DNA methylome landscape upon UV exposure

Project description:Photodamage repair pathways contribute to the accurate maintenance of DNA methylome landscape upon UV exposure

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Photodamage repair pathways contribute to the accurate maintenance of the DNA methylome landscape upon UV exposure.

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Project description:This project was conducted by Nitin S. Baliga of the Halobacterium group at the Institute for Systems Biology in collaboration with Jocelyne Diruggiero of University of Maryland. Halobacterium NRC-1 cell pellets were resuspended in an isotonic buffer at a low density, placed on ice and exposed to 200J/m2 of UV-C irradiation, returned to the growth medium and allowed to recover at 42oC in dark (D) or light (L). Total RNA was collected from each sample at 30 minutes (D30 and L30) and 60 minutes (L60 and D60). The control (C) was processed in a manner to identical to L60 except that it did not suffer any UV-C insult. The reference RNA was prepared from an aliquot of the same culture just prior to UV-C irradiation. Therefore the reference was also exposed to initial perturbations prior to UV-C irradiation such as change of growth medium to buffer and change of temperature from 42oC to ice. Keywords = ISB Keywords = Halobacterium NRC-1 Keywords = UV repair Keywords = ultraviolet radiation Keywords = stress response Keywords: other

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mRNA microarray analysis on young adults C.elegans exposed to UV, TiO2 and UV+TiO2

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The histone demethylase JMJ27 acts during the UV-induced modulation of H3K9me2 landscape and facilitates photodamage repair

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Transcript profiling of three genotypes by ultraviolet-B (UV-B) radiation

Project description:Due to ever increasing environmental deterioration it is likely that influx of solar UV-B radiation (280-320 nm) will increase further due to the depletion of stratospheric ozone. Given this fact it becomes essential that we better understand both rapid and adaptive responses of plants to UV-B imposed stress. Here we compare the transcriptmic responses of wild type Arabidopsis to that of Arabidopsis mutants impaired in flavonoid (TRANSPARENT TESTA4 [tt4] or sinapoyl-malate (sinapoylglucose accumulator 1 [sng1]) biosynthesis, to a short, 24h exposure to this photo-oxidative stress. In control experiments we subjected the same genotypes to 24h treatments of continuous light.

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