Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Rapid nutritional regulation of oligodendrocytes of the adult hypothalamic median eminence revealed by single-cell transcriptomic analysis

ABSTRACT: The median eminence (ME) of the hypothalamus is a structure that rapidly adapts to nutrient availability. We have used single-cell RNA sequencing to characterize the transcriptional profiles of cells specifically in the median eminence in the fasted and refed states. In our study, we focus on characterizing the oligodendrocyte population of the ME, and identifying how subpopulations respond to nutrient availability.

ORGANISM(S): Mus musculus

PROVIDER: GSE133890 | GEO | 2021/06/30

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

A Molecular Census of Arcuate Hypothalamus and Median Eminence Cell Types

Project description:Drop-seq and single cell sequencing of mouse arcuate nucleus and median eminence

2017-02-06 | GSE90806 | GEO

A Molecular Census of Arcuate Hypothalamus and Median Eminence Cell Types

Project description:Drop-seq and single cell sequencing of mouse arcuate nucleus and median eminence. Please see below link for searchable cluster-based gene expression.

2017-01-31 | GSE93374 | GEO

Rapid nutritional regulation of oligodendrocytes of the adult hypothalamic median eminence revealed by single-cell transcriptomic analysis

Project description:Rapid nutritional regulation of oligodendrocytes of the adult hypothalamic median eminence revealed by single-cell transcriptomic analysis

| PRJNA552889 | ENA

Single-cell Transcriptomic Profiling of the Hypothalamic Median Eminence during Aging

Project description:Single-cell Transcriptomic Profiling of the Hypothalamic Median Eminence during Aging

| PRJNA743854 | ENA

Single-cell Transcriptomic Profiling of the Hypothalamic Median Eminence during Aging

Project description:Aging is a slow and progressive natural process that compromises the normal functions of cells, tissues, organs and systems. The aging of the hypothalamic median eminence (ME), a structural gate linking neural and endocrine systems, may impair hormone release, energy homeostasis and central sensing of circulating molecules, leading to systemic and reproductive aging. However, the molecular and cellular features of ME aging remain largely unknown. Here we describe the transcriptional landscape of young and middle-aged mouse ME at single-cell resolution, revealing the common and cell-type-specific transcriptional changes with age. The transcriptional changes in cell-intrinsic programs, cell-cell crosstalk and cell-extrinsic factors highlight five molecular features of ME aging and also implicate several potentially druggable targets at cellular, signaling and molecular levels. Importantly, our results suggest that vascular and leptomeningeal cells may lead the asynchronized aging process among diverse cell types and drive local inflammation and cellular senescence via a unique secretome. Together, our study uncovers how intrinsic and extrinsic features of each cell type in the hypothalamic ME are changed by the aging process, which will facilitate our understanding of brain aging and provide clues for efficient anti-aging intervention at the middle-aged stage.

2022-02-03 | GSE179442 | GEO

Adult Neurogenesis of the Median Eminence Contributes to Structural Reconstruction and Recovery of Body Fluid Metabolism in Hypothalamic Self-Repair

Project description:Purpose: To investigate the underlying regulatory mechanism of neurobiology induced by hypothalamic injury from basic gene expression profile, we extracted RNA from hypothalamic median eminence (ME) RNA sequencing (RNA-seq), at day 1 after control and PEL surgery. Results: We identified 811 differentially expressed genes (DEGs, defined as adjust p value < 0.05) between PEL_1d (day 1 after PEL) and control group. Conclusions:We revealed that RNA-seq based transcriptome characterization would expedite genetic network analyses of hypothalamic ME and permit the dissection of complex biologic functions during the process of central diabetes insipidus after PEL surgery.

2024-01-01 | GSE187602 | GEO

Nutritional regulation of oligodendrocyte differentiation regulates perineuronal net remodeling in the median eminence.

Project description:The mediobasal hypothalamus (MBH; arcuate nucleus of the hypothalamus [ARH] and median eminence [ME]) is a key nutrient sensing site for the production of the complex homeostatic feedback responses required for the maintenance of energy balance. Here, we show that refeeding after an overnight fast rapidly triggers proliferation and differentiation of oligodendrocyte progenitors, leading to the production of new oligodendrocytes in the ME specifically. During this nutritional paradigm, ME perineuronal nets (PNNs), emerging regulators of ARH metabolic functions, are rapidly remodeled, and this process requires myelin regulatory factor (Myrf) in oligodendrocyte progenitors. In genetically obese ob/ob mice, nutritional regulations of ME oligodendrocyte differentiation and PNN remodeling are blunted, and enzymatic digestion of local PNN increases food intake and weight gain. We conclude that MBH PNNs are required for the maintenance of energy balance in lean mice and are remodeled in the adult ME by the nutritional control of oligodendrocyte differentiation.

| S-EPMC8293628 | biostudies-literature

Adult Neurogenesis of the Median Eminence Contributes to Structural Reconstruction and Recovery of Body Fluid Metabolism in Hypothalamic Self-Repair

Project description:Adult Neurogenesis of the Median Eminence Contributes to Structural Reconstruction and Recovery of Body Fluid Metabolism in Hypothalamic Self-Repair

| PRJNA777613 | ENA

Hepatic gene expression profiles during fed–fasted–refed state in mice [mRNA-seq]

Project description:Regulation of nutrient status during fasting and refeeding plays an important role in maintaining metabolic homeostasis in the liver. Thus, we investigated the impact of the physiological fed–fasted–refed cycle on hepatic gene expression in nutrient-sensitive mice. We performed transcriptomic analysis of liver samples in fed, fasted and refed groups of mice. Through mRNA-sequencing (RNA-Seq) and miRNA-Seq, we compared fasted and fed states (fasted versus fed cohort) as well as refed and fasted states (refed versus fasted cohort) to detect dynamic alterations of hepatic mRNA– miRNA expression during the fed–fasted–refed cycle

2023-02-24 | GSE225695 | GEO

Hepatic gene expression profiles during fed–fasted–refed state in mice [miRNA-seq]

2023-02-24 | GSE225696 | GEO