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Ketone body signaling mediates intestinal stem cell homeostasis and adaptation to diet

ABSTRACT: Little is known about how metabolites couple tissue stem function with physiology. Here we show that in the mammalian small intestine, the expression of Hmgcs2 (3-hydroxy-3-methylglutaryl-CoA synthetase 2)—the gene encoding the rate-limiting enzyme in the production of ketone bodies, including beta-hydroxybutyrate (ßOHB)—distinguishes the self-renewing Lgr5+ stem cells (ISCs) from differentiated cell types. Hmgcs2 loss depletes ßOHB levels in Lgr5+ ISCs and skews their differentiation towards secretory cell fates, which can be rescued by exogenous ßOHB and class I histone deacetylases (HDACs) inhibitor treatment. Mechanistically, ßOHB acts by inhibiting HDACs to reinforce Notch signaling in ISCs, thereby instructing self-renewal and lineage decisions. Notably, while a low glucose ketogenic diet elevates ISC function and post-injury regeneration through ßOHB-mediated Notch signaling, a glucose supplemented diet has the opposite effects. These findings reveal how control of ßOHB-activated signaling in ISCs by diet helps to fine-tune stem cell adaptation in homeostasis and injury.

ORGANISM(S): Mus musculus

PROVIDER: GSE134044 | GEO | 2019/10/01

REPOSITORIES: GEO

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