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Profiling the DNA methylation pattern in B3GALT5 knockout hESCs cultured in basal and 2iLAF media

ABSTRACT: We analyzed the DNA methylation pattern in B3GALT5-Knockout H9 cells (B3GALT5-KO) relative to wild type H9 cells (WT) by whole-genome bisulfite sequencing. Collectively, our results reveal that B3GALT5-Knockout cells cultured in 2iLAF condition represent naïve-specific characteristics as well as WT cells convert to naïve state in 5iLAF condition. These findings support that loss of B3GALT5 which result in depletion of primed-specific GSLs not only do not have influence on maintain pluripotency and ability to specific lineage differentiation but also could promotes naive pluripotency generation in hESCs.

ORGANISM(S): Homo sapiens

PROVIDER: GSE134193 | GEO | 2020/10/06

REPOSITORIES: GEO

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