Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profile in porcine ovarian granulosa cells during short-term in vitro culture

ABSTRACT: The porcine ovarian granulosa cells, when analysed during in vitro cultures, may provide new interesting data on the processes associated with in vitro culture of porcine oocytes, as well as assisted reproduction and in vitro fertilisation processes used in the livestock industry. Porcine ovarian granulosa cells were obtained from 43 crossbred Landrace gilts with a median age of 170 days and weight of 98 kg after slaugheter. The cells were harvested after 0h, 48h, 96h and 144h after begining of culture and submited to RNA isolation and microarray analisys. In this study, we demonstrated the gene expression profile of short term primary cultured porcine ovarian granulosa cells.

ORGANISM(S): Sus scrofa Sus scrofa domesticus

PROVIDER: GSE134361 | GEO | 2019/07/17

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Gene expression profile in porcine ovarian granulosa cells during short-term in vitro culture

Project description:Gene expression profile in porcine ovarian granulosa cells during short-term in vitro culture

| PRJNA554853 | ENA

Project description:Small RNA Sequencing of porcine ovarian granulosa cells

| PRJNA813031 | ENA

Project description:Fenoxaprop-ethyl exposure in porcine ovarian granulosa cells

| PRJNA762102 | ENA

Proteomic Analysis of Healthy and Atretic Porcine Follicular Granulosa Cells

Project description:Proteomic Analysis of Healthy and Atretic Porcine Follicular Granulosa Cells

2020-08-25 | PXD021092 |

Transcriptional profiling during fetal skeletal muscle development of Piau and Yorkshire-Landrace crossbred pigs

Project description:The aim of this study was to identify differentially expressed genes and pathways in longissimus dorsi (LD) of pigs at 40 and 70 d of gestation (stages encompassing the transition from primary to secondary fiber formation) in U.S. commercial crossbred pigs (Yorkshire x Landrace) and Brazilian native Piau pigs. We confirmed the expression patterns for a subset of genes by qRT-PCR. Pathway analysis revealed functionally related genes, and indicated commonalities and differences between the breed types and developmental ages evaluated. Results from qRT-PCR analysis confirmed the expression patterns observed on the array for most of the genes tested (85%). This study reveals transcriptional profiles in LD at 40 and 70 d gestation for commercial and Piau pigs, which helps elucidate phenotypic differences between these breed types. This study utilized the Swine Protein-Annotated Oligonucleotide Microarray which contains 20,400 70-mer oligonucleotides (http://www.pigoligoarray.org). Total RNA was isolated from fetuses obtained from gilts at each gestational age (n=3 crossbred gilts; n=4 Piau gilts) and RNA from 3 fetuses per litter was pooled. Samples were evaluated with a connected loop design using 13 slides such that six breed comparisons and seven age comparisons were performed. Fluorescence intensity data was LOESS normalized and analyzed with a mixed model.

2010-04-22 | E-GEOD-21412 | biostudies-arrayexpress

Dietary intake of xylose impacts the transcriptome and proteome of tissues involved in xylose metabolism in swine

Project description:We investigated the transcriptomics and proteomics profiling between different xylose supplementation diets (0% xylose-free vs. 8% xylose (8 g D-xylose/kg)) in swine. We used RNA-seq and proteomics data from kidney, jejunum, liver, and muscle from two replicates of 12 crossbred gilts each to provide 24 animals (12 per dietary 100 treatment; L337 × Camborough, PIC Inc., Hendersonville, TN).

2023-06-13 | GSE233123 | GEO

High-throughput RNA sequencing of human preovulatory cumulus and mural granulosa cells (mRNA)

Project description:The granulosa cells in the mammalian ovarian follicle respond to gonadotropin signalling and are involved in the processes of folliculogenesis and oocyte maturation. Studies on gene expression and regulation in human granulosa cells are of interest due to their potential for estimating the oocyte viability and IVF success. The current study determined the mRNA profile by deep sequencing of the two intrafollicular somatic cell types: mural and cumulus granulosa cells isolated from women undergoing controlled ovarian stimulation and in vitro fertilization. Paired cumulus and mural granulosa samples were analysed from 3 women participating in IVF procedure. Differential gene expression study was performed. The identified gene expression profile was also used for predicting targets for miRNAs that were also identified from the same samples (GSE46489).

2013-08-05 | E-GEOD-46490 | biostudies-arrayexpress

High-throughput RNA sequencing of human preovulatory cumulus and mural granulosa cells (mRNA)

2013-08-05 | GSE46490 | GEO

Directed Differentiation of Human iPSCs to Functional Ovarian Granulosa-Like Cells via Transcription Factor Overexpression [scRNA-seq]

Project description:An in vitro model of human ovarian follicles would greatly benefit the study of female reproduction. Ovarian development requires the combination of germ cells and their supporting somatic cells, known as granulosa cells. Whereas efficient protocols exist for generating human primordial germ cell-like cells (hPGCLCs) from human iPSCs, a method of generating granulosa cells has been elusive. Here we report that simultaneous overexpression of two transcription factors (TFs) can direct the differentiation of human iPSCs to granulosa-like cells. We elucidate the regulatory effects of several granulosa-related TFs, and establish that overexpression of NR5A1 and either RUNX1 or RUNX2 is necessary and sufficient to generate granulosa-like cells. Our granulosa-like cells have transcriptomes similar to human fetal ovarian cells, and recapitulate key ovarian phenotypes including follicle formation and steroidogenesis. When aggregated with hPGCLCs, our cells form ovary-like organoids (ovaroids) and support hPGCLC development from the premigratory to the gonadal stage as measured by induction of DAZL expression. This model system will provide unique opportunities for studying human ovarian biology, and may enable the development of therapies for female reproductive health.

2023-02-21 | GSE213462 | GEO

Directed Differentiation of Human iPSCs to Functional Ovarian Granulosa-Like Cells via Transcription Factor Overexpression [RNA-seq]

2023-02-21 | GSE213156 | GEO