Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mitigating antagonism between transcription and proliferation allows near-deterministic cellular reprogramming (RNAPII ChIP)

ABSTRACT: RNA sequencing analysis of Hb9::GFP mouse embryonic fibroblasts infected with control or reprogramming transcription factors at 4 dpi, and Hb9::GFP+ mouse induced motor neurons generated using transcription factor overexpression. The goal of this study is to examine the transcriptional path of reprogramming cells to identify molecular mechanisms and populations of highly efficient reprogramming.

ORGANISM(S): Mus musculus

PROVIDER: GSE134729 | GEO | 2019/07/24

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Mitigating antagonism between transcription and proliferation allows near-deterministic cellular reprogramming (iMN)

Project description:RNA sequencing analysis of Hb9::GFP mouse embryonic fibroblasts infected with control or reprogramming transcription factors at 4 dpi, and Hb9::GFP+ mouse induced motor neurons generated using transcription factor overexpression. The goal of this study is to examine the transcriptional path of reprogramming cells to identify molecular mechanisms and populations of highly efficient reprogramming.

2019-07-24 | GSE134730 | GEO

Mitigating antagonism between transcription and proliferation allows near-deterministic cellular reprogramming (CNN)

2019-07-24 | GSE134728 | GEO

Mitigating antagonism between transcription and proliferation allows near-deterministic cellular reprogramming (Single Cell)

2019-07-24 | GSE134731 | GEO

Mitigating Antagonism between Transcription and Proliferation Allows Near-Deterministic Cellular Reprogramming.

Project description:Although cellular reprogramming enables the generation of new cell types for disease modeling and regenerative therapies, reprogramming remains a rare cellular event. By examining reprogramming of fibroblasts into motor neurons and multiple other somatic lineages, we find that epigenetic barriers to conversion can be overcome by endowing cells with the ability to mitigate an inherent antagonism between transcription and DNA replication. We show that transcription factor overexpression induces unusually high rates of transcription and that sustaining hypertranscription and transgene expression in hyperproliferative cells early in reprogramming is critical for successful lineage conversion. However, hypertranscription impedes DNA replication and cell proliferation, processes that facilitate reprogramming. We identify a chemical and genetic cocktail that dramatically increases the number of cells capable of simultaneous hypertranscription and hyperproliferation by activating topoisomerases. Further, we show that hypertranscribing, hyperproliferating cells reprogram at 100-fold higher, near-deterministic rates. Therefore, relaxing biophysical constraints overcomes molecular barriers to cellular reprogramming.

| S-EPMC6778044 | biostudies-literature

Mitigating antagonism between transcription and proliferation allows near-deterministic cellular reprogramming

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-07-24 | GSE134732 | GEO

Mitigating antagonism between transcription and proliferation allows near-deterministic cellular reprogramming (RNAPII ChIP)

Project description:Mitigating antagonism between transcription and proliferation allows near-deterministic cellular reprogramming (RNAPII ChIP)

| PRJNA556317 | ENA

Mitigating antagonism between transcription and proliferation allows near-deterministic cellular reprogramming

Project description:Mitigating antagonism between transcription and proliferation allows near-deterministic cellular reprogramming

| PRJNA556313 | ENA

Mitigating antagonism between transcription and proliferation allows near-deterministic cellular reprogramming (CNN)

Project description:Mitigating antagonism between transcription and proliferation allows near-deterministic cellular reprogramming (CNN)

| PRJNA556316 | ENA

Mitigating antagonism between transcription and proliferation allows near-deterministic cellular reprogramming (iMN)

Project description:Mitigating antagonism between transcription and proliferation allows near-deterministic cellular reprogramming (iMN)

| PRJNA556318 | ENA

Mitigating antagonism between transcription and proliferation allows near-deterministic cellular reprogramming (Single Cell)

Project description:Mitigating antagonism between transcription and proliferation allows near-deterministic cellular reprogramming (Single Cell)

| PRJNA556319 | ENA

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data