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Comparative gene expression profiling of human primary endotheliocytes cultivated on polyurethane-based electrospun 3D matrices and natural decellularized vein

ABSTRACT: We have evaluated the functional state of HUVEC cultivated on the surface of autologous decellularized human umbilical vein and electrospun polyurethane-based matrices by NGS gene expression profiling

ORGANISM(S): Homo sapiens

PROVIDER: GSE134909 | GEO | 2019/07/26

REPOSITORIES: GEO

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Comparative gene expression profiling of human primary endotheliocytes cultivated on polyurethane-based electrospun 3D matrices and natural decellularized vein

Project description:Comparative gene expression profiling of human primary endotheliocytes cultivated on polyurethane-based electrospun 3D matrices and natural decellularized vein

| PRJNA556726 | ENA

In search for materials able to be colonized by a normal endothelium: сharacterization and NGS gene expression profiling of human primary endotheliocytes cultivated on electrospun 3D matrices

Project description:Endothelization of the luminal surface of vascular grafts is required for their long-term functioning. In order to select the best surface we have cultivated HUVEC on different 3D matrices and assessed the efficacy of cell proliferation and the changes in the gene expression profiles. 3D matrices were produced by electrospinning from solutions of poly(D,L-lactide-co-glycolide) (PLGA), polycaprolactone (PCL) and blends of PCL with gelatin (PCL-Gl) and glutaraldehyde-treated PCL-GL (PCL-Gl-glu) in hexafluoroisopropanol. Gene expression profiling was executed using Illumina HiSeq2500 platform.

2018-11-29 | GSE123045 | GEO

Proteomic analysi of decellularized porcine pancreas

Project description:The extracellular matrix is an important part of the cellular microenvironment regulating various cellular functions. Decellularized matrices present a valuable strategy for recreating the cellular niche in vitro and implementing matrix signaling in cell culture models. However, the tissue-specificity of decellularized matrices is little considered in most in vitro models. Here, we present the generation of a porcine-derived pancreas-specific decellularized extracellular matrix scaffold (d-PanMa) and show the impact of scaffold-specific cues on stem cell culture. The proteomic analysis in this work compare the proteome of porcine pancreas (PanMa), intestine (SISser) and lung (lungMa) both in its native and decellularized form. The analysis verify the enrichment of important matrisome proteins in the decellular matrices and provide a detailed map of the protein composition and abundance across the matrices.

2020-03-23 | PXD014356 | Pride

Delineation of in vitro chondrogenesis of human synovial stem cells following preconditioning with decellularized matrix

Project description:As a tissue-specific stem cell for chondrogenesis, synovium-derived stem cells (SDSCs) are a promising cell source for cartilage repair. However, a small biopsy can only provide a limited number of cells. Cell senescence from both in vitro expansion and donor age presents a big challenge for stem cell based cartilage regeneration. Here we found that expansion on decellularized extracellular matrix (dECM) full of three-dimensional nanostructured fibers provided SDSCs with unique surface profiles, low elasticity but large volume as well as fibroblast-like shape. dECM expanded SDSCs yielded large pellets with intensive staining of type II collagen and sulfated GAGs, which was supported by both biochemical data and real-time PCR results. Our results also hint at lower levels of inflammatory genes and how they might be responsible for enhanced chondrogenic differentiation in dECM expanded SDSCs. Despite an increase of type X collagen in chondrogenically induced cells, dECM expanded cells had significantly lower potential for endochondral bone formation. Both Wnt and MAPK signals were actively involved in both expansion and chondrogenic induction of dECM expanded cells. dECM expanded human SDSCs could be a potential cell source for autologous cartilage repair Adult human synovial fibroblasts (4 donors, two male and two female, average 43 years old, all had no known joint disease) were grown for one passage on plastic flasks (P cells) or plastic flasks pre-coated with decellularized extracellular matrix (E cells). Aliquots were centrifuged and pellets cultured for 35 days in serum-free chondrogenic medium (P pellet or E pellet). There are no replicates.

2015-06-04 | E-GEOD-69531 | biostudies-arrayexpress

Next Generation Sequencing on HMEC-1 or HUVEC residing on SOFT or STIFF matrices

Project description:Purpose: Endothelial cells respond to changes in subendothelial stiffness altering their proliferation, migration and barrier integrity but whether that is due to transcriptional reprogramming was largely unknown. Using RNA-Sequencing, we performed gene expression profiling for two endothelial cell types grown on soft or stiff matrices: primary human umbilical vein endothelial cells (HUVEC) and immortalized human microvascular endothelial cells (HMEC-1), to understand whether subendothelial stiffness-dependent changes in endothelial cell mechanics are due to transcriptional regulation. Methods and Results: By analyzing the differentially expressed genes between all samples we found that endothelial cell type rather that subendothelial stiffness is the primary determinant of the endothelial cell transcriptome. Both cell types respond to changes in their subendothelial stiffness by increasing the traction stresses they exert on stiffer as opposed to softer matrices, however it is apparently not the endothelial cell transcriptome that regulates this universal biomechanical response to subendothelial stiffness. Only a handful of genes were differentially expressed in each cell type in a stiffness-dependent manner, and none were shared between the two cell types examined. In contrast, thousands of genes were differentially regulated in HUVEC as compared to HMEC-1. HUVEC (but not HMEC-1) upregulate expression of TGF-2 on stiffer matrices, and also enhance their endogenous TGF-2 expression and their cell-matrix traction stresses in response to application of exogenous TGF-2. Conclusions: Altogether, these findings provide insights into the relationship between subendothelial stiffness, endothelial mechanics and variation of the transcriptome between distinct endothelial cell types, and reveal that subendothelial stiffness while critically impacting endothelial cell mechanics is minimally altering their transcriptome.

2019-07-31 | GSE135123 | GEO

In search for materials able to be colonized by a normal endothelium: сharacterization and NGS gene expression profiling of human primary endotheliocytes cultivated on electrospun 3D matrices

Project description:In search for materials able to be colonized by a normal endothelium: сharacterization and NGS gene expression profiling of human primary endotheliocytes cultivated on electrospun 3D matrices

| PRJNA507382 | ENA

Cell-derived decellularized extracellular matrices.

Project description:The ability to create cell-derived decellularized matrices in a dish gives researchers the opportunity to possess a bioactive, biocompatible material made up of fibrillar proteins and other factors that recapitulates key features of the native structure and composition of in vivo microenvironments. By using cells in a culture system to provide a natural ECM, decellularization allows for a high degree of customization through the introduction of selected proteins and soluble factors. The culture system, culture medium, cell types, and physical environments can be varied to provide specialized ECMs for wide-ranging applications to study cell-ECM signaling, cell migration, cell differentiation, and tissue engineering purposes. This chapter describes a procedure for performing a detergent and high pH-based extraction that leaves the native, cell-assembled ECM intact while removing cellular materials. We address common evaluation methods for assessing the ECM and its composition as well as potential uses for a decellularized ECM.

| S-EPMC5995326 | biostudies-literature

The gene expression profile of rat hepatocytes grown on decelllularized liver

Project description:The objective of this study was to determine the gene expression profile of rat hepatocytes grown on decellularized rat liver matrices as a function of time.

2017-12-26 | GSE107274 | GEO

Next Generation Sequencing on HMEC-1 or HUVEC residing on SOFT or STIFF matrices

Project description:Next Generation Sequencing on HMEC-1 or HUVEC residing on SOFT or STIFF matrices

| PRJNA557476 | ENA

Extracellular matrix hydrogel derived from decellularized tissues enables endoderm organoids culture

Project description:Organoids have huge therapeutic potential and are increasingly opening up new avenues within regenerative medicine. However, the clinical application is greatly limited by the lack of effective GMP-complaint systems for organoid expansion in culture. Here, we envisage that the use of extracellular matrix hydrogels derived from decellularized tissues (DT) can provide the instructive native environment necessary to direct cell growth. These gels possess the biochemical signature of tissue-specific ECM, and have the potential for clinical translation due to their natural, non-carcinogenic origin. We show that gels developed from decellularized porcine small intestinal mucosa have similar physiological range and mechanical properties to commercially available gels. ECM-derived gels enable formation and growth of endoderm-derived organoids and, moreover, supports in vivo organoid growth. The development of these ECM-derived hydrogels opens up the potential for human organoids to be used clinically.

2019-11-05 | GSE135108 | GEO

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