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Identification of genes responsive to hyperthermia in human leukemia U937 cells

ABSTRACT: Hyperthermia (41˚C <) is widely used to treat patients with various cancers. Here, the effects of hyperthermia (42˚C for 90 min) on the gene expression in human lymphoma U937 cells were investigated using an Affymetrix GeneChip system. The cells were treated with hyperthermia (42°C for 90 min) and followed by incubation for 0, 1, 3 or 6 h at 37°C. The percentage of DNA fragmentation was 7.5 ± 0.9 (mean ± SD), 10.1 ± 0.2, and 17.3 ± 2.3 at the incubation periods of 1, 3, and 6 h, respectively. Of approximately 47,000 probe sets analyzed, the hyperthermia down-regulated 4,214 probe sets and up-regulated 1,334 by a factor 2.0 or greater.

ORGANISM(S): Homo sapiens

PROVIDER: GSE13503 | GEO | 2008/11/11

SECONDARY ACCESSION(S): PRJNA110103

REPOSITORIES: GEO

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Transcription profiling of human leukemia U937 cells response to hyperthermia

Project description:Hyperthermia (41ËC <) is widely used to treat patients with various cancers. Here, the effects of hyperthermia (42ËC for 90 min) on the gene expression in human lymphoma U937 cells were investigated using an Affymetrix GeneChip system. The cells were treated with hyperthermia (42Â°C for 90 min) and followed by incubation for 0, 1, 3 or 6 h at 37Â°C. The percentage of DNA fragmentation was 7.5 Â± 0.9 (mean Â± SD), 10.1 Â± 0.2, and 17.3 Â± 2.3 at the incubation periods of 1, 3, and 6 h, respectively. Of approximately 47,000 probe sets analyzed, the hyperthermia down-regulated 4,214 probe sets and up-regulated 1,334 by a factor 2.0 or greater. Experiment Overall Design: U937 cells, a human lymphoma cell line, were treated with hyperthermia (42Â°C for 90 min) and followed by incubation for 0, 1, 3 or 6 h at 37Â°C. Non-treated cells were served as control. Total RNA samples were prepared from the cells. Gene expression was analyzed by an Affymetrix GeneChipÂ® system with a Human Genome U133-plus 2.0 array for analysis of over 47,000 transcripts. Sample preparation for array hybridization was carried out as described in the manufacturerâs instructions.

2008-11-15 | E-GEOD-13503 | biostudies-arrayexpress

Gene profiling of the cell death induced by heat stress in HSC-3 human oral squamous carcinoma cells

Project description:Hyperthermia is widely used to treat patients with various cancers. The 42.5˚C is well known as inflection point of hyperthermia and generally up to 42˚C of hyperthermia is used in clinical case to combine with other therapy. Here, the effects of heat stress at 42 or 44˚C for 90 min on the gene expression in HSC-3 human oral squamous carcinoma cells were investigated using an Affymetrix GeneChip system. The cells were treated with heat stress (42 or 44°C for 90 min) and followed by incubation for 0, 6, or 12 h at 37°C. The percentage of cell death was 5.0 ± 1.5 (mean ± SD) at 42°C for 12 h and 17.4 ± 0.6 at 44°C for 12 h. Of approximately 47,000 probe sets analyzed, many genes that were differentially expressed by a factor 2.0 or greater were identified in the cells treated with heat stress at 42 and 44°C.

2010-10-20 | GSE24783 | GEO

Gene profiling of the cell death induced by heat stress in HSC-3 human oral squamous carcinoma cells

Project description:Hyperthermia is widely used to treat patients with various cancers. The 42.5ËC is well known as inflection point of hyperthermia and generally up to 42ËC of hyperthermia is used in clinical case to combine with other therapy. Here, the effects of heat stress at 42 or 44ËC for 90 min on the gene expression in HSC-3 human oral squamous carcinoma cells were investigated using an Affymetrix GeneChip system. The cells were treated with heat stress (42 or 44Â°C for 90 min) and followed by incubation for 0, 6, or 12 h at 37Â°C. The percentage of cell death was 5.0 Â± 1.5 (mean Â± SD) at 42Â°C for 12 h and 17.4 Â± 0.6 at 44Â°C for 12 h. Of approximately 47,000 probe sets analyzed, many genes that were differentially expressed by a factor 2.0 or greater were identified in the cells treated with heat stress at 42 and 44Â°C. HSC-3 human oral squamous carcinoma cells were treated with heat stress (42 or 44Â°C for 90 min) and followed by incubation for 0, 6, or 12 h at 37Â°C. Non-treated cells were served as control. Total RNA samples were prepared from the cells. Gene expression was analyzed by an Affymetrix GeneChipÂ® system with a Human Genome U133-plus 2.0 array for analysis of over 47,000 transcripts. Sample preparation for array hybridization was carried out as described in the manufacturerâs instructions.

2010-10-20 | E-GEOD-24783 | biostudies-arrayexpress

Gene profiling of apoptosis induced by heat stress in U937 human lymphoma cells

2010-08-11 | GSE23405 | GEO

Transcription profiling of apoptosis induced by heat stress in U937 human lymphoma cells

Project description:Hyperthermia is widely used to treat patients with various cancers. 42.5˚C is well known as the inflection point of hyperthermia and generally up to 42˚C of hyperthermia is used in clinical cases combined with other therapies. Here, the effects of heat stress at 42 or 44˚C for 15 min on the gene expression in human lymphoma U937 cells were investigated using an Affymetrix GeneChip system. The cells were treated with heat stress (42 or 44°C for 15 min), followed by incubation for 0, 1, 3 or 6 h at 37°C. The percentage of DNA fragmentation was 8.4 ± 2.2 (mean ± SD) at 42°C for 6 h and 21.0 ± 2.0 at 44°C for 6 h. Of approximately 47,000 probe sets analyzed, many genes that were differentially expressed by a factor 2.0 or greater were identified in the cells treated with heat stress at 42 and 44°C. U937 cells, a human lymphoma cell line, were treated with heat stress (42 or 44°C for 15 min), followed by incubation for 0, 1, 3 or 6 h at 37°C. Non-treated cells served as the control. Total RNA samples were prepared from the cells. Gene expression was analyzed by an Affymetrix GeneChip® system with a Human Genome U133-plus 2.0 array for analysis of over 47,000 transcripts. Sample preparation for array hybridization was carried out as described in the manufacturer’s instruction

2010-08-11 | E-GEOD-23405 | biostudies-arrayexpress

Identification of genes responsive to the combination of Sonazoid and ultrasound in human leukemia U937 cells

2009-05-16 | GSE16118 | GEO

Transcription profiling of human leukemia U937 cells response to mild hypothermia

Project description:The rationale underlying hyperthermia is the fact that temperatures over 42.5ËC are highly cytotoxic to tumor cells. On the other hand, although mild hyperthermia at a range from 39 to 41ËC alone did not induce cytotoxicity in tumor cells, mild hyperthermia is reported to show a synergism with radiotherapy and anti-cancer drugs. Here, the effects of mild hyperthermia (41ËC for 30 min) on the gene expression in human lymphoma U937 cells were investigated using by an Affymetrix GeneChip system. Although the cells treated with the mild hyperthermia did not induce apoptosis, a significant increase in protein levels of heat shock proteins, Hsp40 and Hsp70, was observed following activation of heat shock factor-1. At 3 h post-treatment, 938 probe sets that were differentially expressed by >1.5-fold were identified. Experiment Overall Design: U937 cells, a human lymphoma cell line, were treated with mild hyperthermia (41ËC for 30 min) and followed by incubation for 3 h at 37Â°C. Non-treated cells were served as control. Total RNA samples were prepared from the cells. Gene expression was analyzed by an Affymetrix GeneChipÂ® system with Human Expression Array U133A which was spotted with 22,283 probe sets. Sample preparation for array hybridization was carried out as described in the manufactureâs instructions.

2008-06-11 | E-GEOD-10043 | biostudies-arrayexpress

Comprehensive multi-omics analysis revealed WEE1 as a synergistic lethal target with hyperthermia through CDK super-activation

Project description:To explore the translational expression on mRNA levels after hyperthermia，we exposed OVCAR8 cells to hyperthemia (42℃) for 90 mins.

2023-12-12 | GSE227393 | GEO

Identification of genes responsive to mild hyperthermia in human leukemia U937 cells

Project description:The rationale underlying hyperthermia is the fact that temperatures over 42.5˚C are highly cytotoxic to tumor cells. On the other hand, although mild hyperthermia at a range from 39 to 41˚C alone did not induce cytotoxicity in tumor cells, mild hyperthermia is reported to show a synergism with radiotherapy and anti-cancer drugs. Here, the effects of mild hyperthermia (41˚C for 30 min) on the gene expression in human lymphoma U937 cells were investigated using by an Affymetrix GeneChip system. Although the cells treated with the mild hyperthermia did not induce apoptosis, a significant increase in protein levels of heat shock proteins, Hsp40 and Hsp70, was observed following activation of heat shock factor-1. At 3 h post-treatment, 938 probe sets that were differentially expressed by >1.5-fold were identified. Keywords: mild hyperthermia, gene expression, Human lymphoma U937 cell

2008-01-03 | GSE10043 | GEO

Transcription profiling of human leukemia U937 cells treated with the combination of Sonazoid and ultrasound

Project description:Echo-contrast agents enhance the echogenicity of ultrasound and have been clinically used for diagonosis in current medical fields. Here, the combined effects of Sonazoid, an echo-contrast agent, and ultrasound on the gene expression in human lymphoma U937 cells were investigated using an Affymetrix GeneChip system. The cells were treated with Sonazoid (0.05%; Sonazoid only), ultrasound (0.3 W/cm2 for 1 min; ultrasound only) and the combination of Sonazoid and ultrasound (0.05% Sonazoid plus ultrasound 0.3 W/cm2 for 1 min; Sonazoid + Ultrasound) and followed by incubation for 3 h at 37Â°C. The percentage of DNA fragmentation 6 h after treatment was 5.8 Â± 1.0 (mean Â± SD, n = 3), 6.0 Â± 0.4, 13.5 Â± 1.0, and 18.3 Â± 2.3 in cells treated with control, Sonazoid only, ultrasound only and Sonazoid + Ultrasound, respectively. Of approximately 47,000 probe sets analyzed, probe sets that were differentially expressed by a factor 2.0 or greater were 40, 184 and 144 in cells treated with Sonazoid only, ultrasound only and Sonazoid + Ultrasound, respectively. Experiment Overall Design: U937 cells, a human lymphoma cell line, were treated with Sonazoid (0.05%), ultrasound (0.3 W/cm2 for 1 min) and the combination of Sonazoid and ultrasound (0.05% Sonazoid plus ultrasound 0.3 W/cm2 for 1 min) and followed by incubation for 3 h at 37Â°C. Non-treated cells were served as control. Total RNA samples were prepared from the cells. Gene expression was analyzed by an Affymetrix GeneChipÂ® system with a Human Genome U133-plus 2.0 array for analysis of over 47,000 transcripts. Sample preparation for array hybridization was carried out as described in the manufacturerâ??s instructions.

2009-05-27 | E-GEOD-16118 | biostudies-arrayexpress

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