Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Global transcriptional profiles of abdominal skin epidermis during perinatal period

ABSTRACT: We report global transcriptional alteration and transcriptional heterogeneity of epidermal cells in remodeling mouse abdominal skin during perinatal period. By RNA-sequencing of FACS-isolated interfollicular epidermal (IFE) basal cells from abdominal skin of wildtype (WT) non-pregnant (NP) mice, pregnant mice, and Tbx3 cKO pregnant mice, we revealed pregnancy-associated genes and a key role of Tbx3 in regulating the pregnancy-associated transcriptome. To resolve the transcriptional heterogeneity of epidermal cells at single cell resolution, we performed single cell RNA sequencing of epidermal cells isolated from the abdominal skin of NP mice, pregnant mice, and post-partum mice by using Tbx3creERT2;R26H2B-EGFP mice. By unsupervised evaluation of clustering-based cell identities of total samples on the Seurat platform, we identified 14 main clusters, where the IFE1 cluster was appeared to be enriched in Dpc16 samples, and further revealed the pseudotime differentiation of Tbx3cre labeled cells during perinatal period.

ORGANISM(S): Mus musculus

PROVIDER: GSE136747 | GEO | 2021/02/17

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Global transcriptional profiles of abdominal skin dermis during perinatal period

Project description:We report global transcriptional alteration and transcriptional heterogeneity of dermal cells in remodeling mouse abdominal skin during perinatal period. By single cell RNA sequencing of whole dermal cells isolated from the abdominal skin dermis of non-pregnant (NP) mice, pregnant mice at dpc16, and post-partum mice at dpp42 by using Col1a2creERT2;R26H2B-EGFP mice. By unsupervised evaluation of clustering-based cell identities of total samples on the Seurat platform, we identified 13 main clusters. The second round of unsupervised clustering of each cluster revealed that the vascular cluster exhibits dynamic transcriptome alteration during pregnancy.

2021-02-17 | GSE151212 | GEO

Global transcriptional profiles of abdominal skin dermis during perinatal period

Project description:Global transcriptional profiles of abdominal skin dermis during perinatal period

| PRJNA635131 | ENA

Global transcriptional profiles of abdominal skin epidermis during perinatal period

Project description:Global transcriptional profiles of abdominal skin epidermis during perinatal period

| PRJNA563567 | ENA

The mice uterine gene expression landscape from early to late pregnancy

Project description:To define the vast landscape of mice pregnancy and labor/parturition associated gene expression transitions, wild type FVB mice uterine tissues from the non-pregnant state (NP) and at E6.5, E8.5, E10.5, E15.5 and E17.5 were analysed using gene expression microarray

2018-11-07 | GSE85934 | GEO

Microarray data of epidermal basal cells from ventral or dorsal skin of virgin and pregnant mice

Project description:To identify genes expressed predominantly in the ventral skin epidermal basal cells of pregnant mice, we performed DNA microarray analysis by using FACS-purified epidermal basal cells from ventral skin at 0 and 16 dpc, and dorsal skin at 16 dpc.

2016-08-30 | GSE86157 | GEO

A transcriptomic study of maternal thyroid adaptation to pregnancy in rats

Project description:To identify potential regulators of late pregnancy-dependent thyroid weight increase, thyroid gene expression profiles of non-pregnant (NP) and late pregnant (LP, day 18) rats was analyzed using the RNA-seq approach.

2015-09-23 | GSE73307 | GEO

Transcriptome profile form endometrial biopsy samples of pregnant and non-pregnant cows

Project description:Purpose: characterize the uterine transcriptome profiles of pregnant (P) versus non-pregnant (NP) cows during early pregnancy and attempted to define a potential set of marker genes that can be valuable for predicting pregnancy outcome. Methods: beef cows were synchronized and artificially inseminated at detected estrus. Six days after AI, jugular blood samples and a biopsy from the uterine horn contralateral to the ovary containing the corpus luteum were collected. Based on pregnancy outcome on day 30, samples were retrospectively allocated to the following groups: Pregnant and Non-Pregnant. Both groups had similar plasma progesterone concentrations on D6. Uterine biopsies were submitted to RNA-Seq analysis in a Illumina HiScanSQ platform. Results: The 272,685,768 million filtered reads were mapped to the Bos taurus reference genome and 14,654 genes were analyzed for differential expression between groups. Transcriptome data showed that 216 genes are differently expressed when comparing NP versus P uterine tissue (Padj≤0.1). More specifically, 36 genes were up-regulated in P cows and 180 are up-regulated in NP cows. Conclusions: this study characterized a unique set of genes, expressed in the uterus on 6 days after insemination, that indicate a receptive state leading to pregnancy success. Furthermore, expression of such genes can be used as potential markers to efficiently predict pregnancy success. endometrial mRNA profiles of pregnant and non-pregnant cows were generated by deep sequencing using Illumina HiScanSQ platform BioProject PRJNA268916 SRA Study SRP05036

2015-04-09 | E-GEOD-65117 | biostudies-arrayexpress

Pig liver postnatal vs adult proteome analysis

Project description:During the perinatal period, unique metabolic adaptations support energetic requirements for rapid growth. To gain insight into perinatal adaptations, quantitative proteomics were performed comparing the livers of yorkshire pigs at postnatal day seven and adult. These data revealed differences in the metabolic control of liver function including significant changes in lipid and carbohydrate metabolic pathways. Newborn livers showed an enrichment of proteins in lipid catabolism and gluconeogenesis concomitant with elevated liver carnitine and acylcarnitines levels. Sugar kinases were some of the most dramatically differentially enriched proteins comparing neonatal and adult pigs including galactokinase 1 (Galk1), ketohexokinase (KHK), hexokinase 1 (HK1) and hexokinase 4 (GCK). Interestingly, hexokinase domain containing 1 (HKDC1), an enigmatic fifth hexokinase associated with glucose disturbances in pregnant women was highly enriched in the liver during the prenatal and perinatal periods and continuously declined throughout postnatal development in pigs and mice. These changes were confirmed via Western blot and mRNA expression. These data provide new insights into the developmental and metabolic adaptations in the liver during the transition from the perinatal period to adulthood in multiple mammalian species.

2023-07-17 | PXD037264 | Pride

Transcriptome profile form endometrial biopsy samples of pregnant and non-pregnant cows

2015-04-09 | GSE65117 | GEO

B cells acquire a unique and differential transcriptomic profile during pregnancy in mice

Project description:Pregnancy significantly alters B cells development and function. B cell activation is initiated by antigens specific binding to the BCR, leading to an intricate signaling pathway responsible for B cell survival, proliferation, antigen processing/presentation and antibody production. We performed a genome-wide transcriptome profiling in splenic B cells from pregnant (P) and non-pregnant (NP) mice. We identified 1136 genes with differential expression in B cells from P mice (625 up- and 511 down-regulated) compared to NP animals. In silico analysis showed that B cell activation through BCR-signaling pathway as well as antigen processing/presentation and TLR cascades seems to be lowered during pregnancy. Further RT-qPCR analysis confirmed these data. In addition, B cells from pregnant women stimulated in vitro through BCR in combination with CpG, produced significantly lower levels of cytokines compared to non-pregnant women. Our results suggest that B cells acquire a state of hypo-responsiveness during gestation, probably as part of the maternal immune strategy for fetal tolerance. However, our results also open new avenues to understand why pregnant women are at highest risk for some infections.

2021-07-27 | GSE174290 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data