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BAT Hi-C maps global chromatin interactions in an efficient and economical way

ABSTRACT: We describe the Bridge linker-Alul-Tn5 Hi-C (BAT Hi-C) method, which is a simple and efficient method for delineating chromatin conformational features of mouse embryonic stem (mES) cells and uncover DNA loops. This protocol combines Alul fragmentation and biotinylated linker-mediated proximity ligation to obtain kilobase (kb) resolution with a marked increase in the amount of unique read pairs. The protocol also includes chromatin isolation to reduce background noise and Tn5 tagmentation to cut down on preparation time.

ORGANISM(S): Mus musculus

PROVIDER: GSE136756 | GEO | 2019/09/30

REPOSITORIES: GEO

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