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Effect of NF90 knock-down on whole gene expression in Beta-TC-6 cells, a pancreatic beta cell line

ABSTRACT: We found that knock down of NF90 and its binding factor, NF45, complex leads to retardation of growth in Beta-TC-6 cells, a pancreatic beta cell line. To uncover molecular mechanisms for the growth retardation in Beta-TC-6 cells depleted of NF90-NF45, we performed comprehensive analysis of gene expression using microarray.

ORGANISM(S): Mus musculus

PROVIDER: GSE136794 | GEO | 2019/09/04

REPOSITORIES: GEO

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Effect of NF90-NF45 knock-down on whole gene expression in Beta-TC-6 cells, a pancreatic beta cell line

Project description:We found that knock down of NF90 and its binding factor, NF45, complex leads to retardation of growth in Beta-TC-6 cells, a pancreatic beta cell line. To uncover molecular mechanisms for the growth retardation in Beta-TC-6 cells depleted of NF90-NF45, we performed comprehensive analysis of gene expression using microarray.

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Effect of NF45 knock-down on whole gene expression in Beta-TC-6 cells, a pancreatic beta cell line

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Whole genome expression microarray analysis in quadriceps of wild-type and NF90-NF45 double transgenic mouse

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Effect of NF90-NF45 knock-down on whole gene expression in Beta-TC-6 cells, a pancreatic beta cell line

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MicroRNA (miRNA) expression profile in quadriceps of wild-type (WT) and NF90-NF45 double transgenic (dbTg) mice.

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NF45 and NF90/NF110 coordinately regulate ESC pluripotency and differentiation

Project description:While years of investigation have elucidated many aspects of embryonic stem cell (ESC) regulation, the contributions of post-transcriptional and translational mechanisms to the pluripotency network remain largely unexplored. In particular, little is known in ESCs about the function of RNA binding proteins (RBPs), the protein agents of post-transcriptional regulation. We performed an unbiased RNAi screen of RBPs in an ESC differentiation assay and identified two related genes, NF45 (Ilf2) and NF90/NF110 (Ilf3), whose knockdown promoted differentiation to an epiblast-like state. Characterization of NF45 KO, NF90+NF110 KO, and NF110 KO ESCs showed that loss of NF45 or NF90+NF110 impaired ESC proliferation and led to dysregulated differentiation down embryonic lineages. Additionally, we found that NF45 and NF90/NF110 physically interact and influence the expression of each other at different levels of regulation. Globally across the transcriptome, NF45 KO ESCs and NF90+NF110 KO ESCs show similar expression changes. Moreover, NF90+NF110 RNA immunoprecipitation (RIP)-seq in ESCs suggested that NF90/NF110 directly regulate proliferation, differentiation, and RNA-processing genes. Our data support a model in which NF45, NF90, and NF110 operate in feedback loops that enable them, through both overlapping and independent targets, to help balance the push and pull of pluripotency and differentiation cues.

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Effect of NF90 knock-down on whole gene expression in Beta-TC-6 cells, a pancreatic beta cell line

Project description:Effect of NF90 knock-down on whole gene expression in Beta-TC-6 cells, a pancreatic beta cell line

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MicroRNA (miRNA) expression profile in Huh7 cells treated with siRNA targeting nuclear factor 90 (siNF90)

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