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RNA Seq of HMVEC under hypoxia

ABSTRACT: Purpose: to profile transcriptome changes due to EC-AGO1-deficiency Methods: We had HMVECs subjected to normoxia (20%) or hypoxia (2%) for 0, 12, 24, and 48 hr in biological replicates. Total RNA was extracted with mirVana total RNA isolation kit (Life Technologies). RNA-seq was performed. Results: We found genes involved in pathways promoting angiogenesis, browning, insulin sensitivity to be upregulated, and those promoting inflammation and fibrosis to be decreased in KO compared with WT mice. Conclusions:Our study represents the detailed analysis of Ago1 regulate transcriptomes in HMVEC under Hx. We conclude that RNA-seq based transcriptome characterization would expedite genetic network analyses and permit the dissection of complex biologic functions. To elucidate the molecular changes underlying the observed metabolic phenotype in EC-AGO1-KO mice.

ORGANISM(S): Homo sapiens

PROVIDER: GSE136908 | GEO | 2019/09/06

REPOSITORIES: GEO

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