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Investigation on different gene and small RNA expression of TT19 suppressors under anthocyanin inducing conditions (AIC)

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE137004 | GEO | 2020/04/20

REPOSITORIES: GEO

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Investigation on different gene expression of TT19 suppressors under anthocyanin inducing conditions (AIC) using RNA-seq

Project description:24 samples of total RNA of 8 Arabidopsis lines (col-0, tt19, and six tt19 suppressors in triplicate) which were under AIC (shaking in 3% sucrose for four days under 24hrs of light) was extracted. mRNA libraries were prepared by BGI company and sequenced BGISEQ-500 as 100PE.

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Investigation on different gene and small RNA expression of TT19 suppressors under anthocyanin inducing conditions (AIC)

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Investigation on different small RNA expression of TT19 suppressors under anthocyanin induction condition (AIC) using Small RNA-seq

Project description:To investigate the functions of TRANSPARENT TESTA 19 (TT19) on anthocyanin accumulation and stabilization using forward genetic screening approach. EMS mutagenized tt19 mutant to screen tt19 mutant suppressors that could restore the anthocyanin accumulation and the causative mutations were identified as point mutations in the RDR6/SGS3/DCL4 siRNA biogenesis pathway using whole genome re-sequencing. The idea for RNA-Seq and small RNA-Seq analysis is to illustrate the connection between siRNA biogenesis, TT19, and anthocyanin restoration. RNA's of 4 Arabidopsis lines (col-0, tt19 and two TT19 suppressors, all in triplicate) which were under AIC (3% sucrose shaking four days under 24h light) for four days was extracted. Small RNA libraries were prepared by BGI company and sequenced using BGISEQ-500 as 50SE.

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