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Sex chromosomes affect gene expression of autoantigen specific CD4+ T cells in B6 gonadal males.

ABSTRACT: To determine the role of sex chromsomes in sex differences of autoimmune disease, we used high throughput RNA sequencing to analyze the transciptomes of autoantigen specific CD4+ T cells from XX and XY- mice using the “four core genotypes” mouse model. We found higher expression of several X genes in the XY- genotype compared to XX, suggesting a difference in DNA methylation of these genes between Xp and Xm. In a hypothesis driven targeted approach, we investigated two immunomodulatory X genes, Tlr7 and FoxP3 and found that the promoter regions of both genes had more DNA methylation on the Xp than Xm, and that both genes had higher expression in autoantigen stimulated CD4+ T cells from XY- compared to XX mice. This study provides new insights regarding how sex chromsomes affect gene expression and cause sex differences in autoimmune disease.

ORGANISM(S): Mus musculus

PROVIDER: GSE137793 | GEO | 2019/12/10

REPOSITORIES: GEO

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Sex chromosomes affect gene expression of healthy CD4+ T cells in B6 gonadal females.

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