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Division of Labor between PCNA Loaders in DNA Replication and Sister Chromatid Cohesion Establishment [BrdU-IP-ssSeq, ChIP-ssSeq and eSPAN analysis]

ABSTRACT: Strand Specific PCNA loading by Ctf18-RFC and Rfc1-RFC at Saccharomyces cerevisiae replication forks

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE138055 | GEO | 2020/03/29

REPOSITORIES: GEO

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Division of Labor Between PCNA Loaders in DNA Replication and Sister Chromatid Cohesion Establishment [Affymetrix ChIP-chip]

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Division of Labor between PCNA Loaders in DNA Replication and Sister Chromatid Cohesion Establishment.

Project description:Concomitant with DNA replication, the chromosomal cohesin complex establishes cohesion between newly replicated sister chromatids. Several replication-fork-associated "cohesion establishment factors," including the multifunctional Ctf18-RFC complex, aid this process in as yet unknown ways. Here, we show that Ctf18-RFC's role in sister chromatid cohesion correlates with PCNA loading but is separable from its role in the replication checkpoint. Ctf18-RFC loads PCNA with a slight preference for the leading strand, which is dispensable for DNA replication. Conversely, the canonical Rfc1-RFC complex preferentially loads PCNA onto the lagging strand, which is crucial for DNA replication but dispensable for sister chromatid cohesion. The downstream effector of Ctf18-RFC is cohesin acetylation, which we place toward a late step during replication maturation. Our results suggest that Ctf18-RFC enriches and balances PCNA levels at the replication fork, beyond the needs of DNA replication, to promote establishment of sister chromatid cohesion and possibly other post-replicative processes.

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Division of Labor Between PCNA Loaders in DNA Replication and Sister Chromatid Cohesion Establishment

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-03-28 | GSE138056 | GEO

Division of Labor Between PCNA Loaders in DNA Replication and Sister Chromatid Cohesion Establishment

Project description:Division of Labor Between PCNA Loaders in DNA Replication and Sister Chromatid Cohesion Establishment

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Division of Labor between PCNA Loaders in DNA Replication and Sister Chromatid Cohesion Establishment [BrdU-IP-ssSeq, ChIP-ssSeq and eSPAN analysis]

Project description:Division of Labor between PCNA Loaders in DNA Replication and Sister Chromatid Cohesion Establishment [BrdU-IP-ssSeq, ChIP-ssSeq and eSPAN analysis]

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PCNA promotes context-specific sister chromatid cohesion establishment separate from that of chromatin condensation.

Project description:Cellular genomes undergo various structural changes that include cis tethering (the tethering together of two loci within a single DNA molecule), which promotes chromosome condensation and transcriptional activation, and trans tethering (the tethering together of two DNA molecules), which promotes sister chromatid cohesion and DNA repair. The protein complex termed cohesin promotes both cis and trans forms of DNA tethering, but the extent to which these cohesin functions occur in temporally or spatially defined contexts remains largely unknown. Prior studies indicate that DNA polymerase sliding clamp PCNA recruits cohesin acetyltransferase Eco1, suggesting that sister chromatid cohesion is established in the context of the DNA replication fork. In support of this model, elevated levels of PCNA rescue the temperature growth and cohesion defects exhibited by eco1 mutant cells. Here, we test whether Eco1-dependent chromatin condensation is also promoted in the context of this DNA replication fork component. Our results reveal that overexpressed PCNA does not promote DNA condensation in eco1 mutant cells, even though Smc3 acetylation levels are increased. We further provide evidence that replication fork-associated E3 ligase impacts on Eco1 are more complex that previously described. In combination, the data suggests that Eco1 acetylates Smc3 and thus promotes sister chromatid cohesion in context of the DNA replication fork, whereas a distinct cohesin population participates in chromatin condensation outside the context of the DNA replication fork.

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Division of Labor Between PCNA Loaders in DNA Replication and Sister Chromatid Cohesion Establishment [Affymetrix ChIP-chip]

Project description:Division of Labor Between PCNA Loaders in DNA Replication and Sister Chromatid Cohesion Establishment [Affymetrix ChIP-chip]

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Targeted sister chromatid cohesion by Sir2.

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Sororin actively maintains sister chromatid cohesion.

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Pericentromeric sister chromatid cohesion promotes kinetochore biorientation.

Project description:Accurate chromosome segregation depends on sister kinetochores making bioriented attachments to microtubules from opposite poles. An essential regulator of biorientation is the Ipl1/Aurora B protein kinase that destabilizes improper microtubule-kinetochore attachments. To identify additional biorientation pathways, we performed a systematic genetic analysis between the ipl1-321 allele and all nonessential budding yeast genes. One of the mutants, mcm21Delta, precociously separates pericentromeres and this is associated with a defect in the binding of the Scc2 cohesin-loading factor at the centromere. Strikingly, Mcm21 becomes essential for biorientation when Ipl1 function is reduced, and this appears to be related to its role in pericentromeric cohesion. When pericentromeres are artificially tethered, Mcm21 is no longer needed for biorientation despite decreased Ipl1 activity. Taken together, these data reveal a specific role for pericentromeric linkage in ensuring kinetochore biorientation.

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