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Maintenance of primary hepatocyte functions in vitro by inhibiting mechanical tension-induced YAP activation

ABSTRACT: It has been a long-standing challenge to maintain the functions of hepatocytes in vitro. In the present study, we found that mechanical tension-induced yes-associated protein (Yap) activation triggered hepatocyte dedifferentiation. Alleviation of mechanical tension by confinement of cell spreading was sufficient to inhibit hepatocyte dedifferentiation. Based on this finding, we identified a small molecular cocktail LBDXL through reiterative chemical screening that could maintain hepatocyte functions over the long term and in vivo repopulation capacity by targeting actin polymerization and actomyosin contraction.

ORGANISM(S): Mus musculus

PROVIDER: GSE138158 | GEO | 2019/10/30

REPOSITORIES: GEO

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