High temporal resolution RNA-seq time series of serum-starved T98G human glioblastoma cells responding to serum stimulation

ABSTRACT: The advent of next-generation sequencing revealed extensive transcription outside the boundaries of annotated protein-coding genes, giving rise to tens of thousands of long non-coding RNAs (lncRNAs). The functional relevance of these transcripts remains unclear. Compellingly, lncRNA expression is strongly linked with adjacent protein-coding gene expression, suggesting potential cis-regulatory roles. To investigate whether these regulatory roles exist, we examine in detail the timing of lncRNA expression relative to transcripts of known function. If a causal cis-regulatory relationship exists, lncRNA activation must necessarily precede changes in adjacent target gene expression. Here, we report a high temporal resolution RNA-seq time course of synchronized T98G human glioblastoma cells responding to serum stimulation. Detailed profiling of the expression dynamics of lncRNAs and protein-coding genes in these synchronized transitioning human cells provides insight into the feasibility of broad-scale cis-regulatory roles for lncRNAs.

ORGANISM(S): Homo sapiens

PROVIDER: GSE138662 | GEO | 2022/06/23

REPOSITORIES: GEO