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NicE-seq in Suv39h2 knockdown 8-cell stage

ABSTRACT: Determination of accessible chromatin regions in the 8-cell stage mouse embryo after Suv39h2 knockdown The study aimed to address a potential function of H3K9me3 in early mouse development by assessing its impact on chromatin compaction. The histone methyltransferase responsible for de novo H3K9me3 at fertilization Suv39h2, was knocked down by microinjection of dsRNA targeting Suv39h2 in the early zygote. Embryos were then cultured until the 8-cell stage of development. They were then fixed and chromatin compaction was assessed by NicE-seq in pools of 10 8-cell stage embryos.

ORGANISM(S): Mus musculus

PROVIDER: GSE138686 | GEO | 2020/03/31

REPOSITORIES: GEO

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NicE-seq in Suv39h2 knockdown 8-cell stage

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