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Ectopic Tcf1 expression reprograms exhausted CD8+ T cells to acquire stem cell-like features [CUT&RUN]

ABSTRACT: The goal of this study is to determine the capacity of Tcf1 to reprogram exhausted CD8+ T cells for function restoration. Tex cells elicited by chronic infection contain a CXCR5+Tim3– subset that has stem cell-like self-renewing capacity and expresses an elevated level of Tcf1 (termed Tex-stem), and Tex-stem cells depend on Tcf1 for generation and persistence. We show that ectopic Tcf1 expression profoundly affected the transcriptome and chromatin accessibility of Tex cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE139056 | GEO | 2020/04/30

REPOSITORIES: GEO

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Ectopic Tcf1 expression reprograms exhausted CD8+ T cells to acquire stem cell-like features [ATAC-seq]

Project description:The goal of this study is to determine the capacity of Tcf1 to reprogram exhausted CD8+ T cells for function restoration. Tex cells elicited by chronic infection contain a CXCR5+Tim3– subset that has stem cell-like self-renewing capacity and expresses an elevated level of Tcf1 (termed Tex-stem), and Tex-stem cells depend on Tcf1 for generation and persistence. We show that ectopic Tcf1 expression profoundly affected the chromatin accessibility of Tex cells.

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