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Complement receptor 2/CD21– human naive B cells contain mostly autoreactive unresponsive clones

ABSTRACT: Complement receptor 2–negative (CR2/CD21–) B cells have been found enriched in patients with autoimmune diseases and in common variable immunodeficiency (CVID) patients who are prone to autoimmunity. However, the physiology of CD21–/lo B cells remains poorly characterized. We found that some rheumatoid arthritis (RA) patients also display an increased frequency of CD21–/lo B cells in their blood. A majority of CD21–/lo B cells from RA and CVID patients expressed germline autoreactive antibodies, which recognized nuclear and cytoplasmic structures. In addition, these B cells were unable to induce calcium flux, become activated, or proliferate in response to B-cell receptor and/or CD40 triggering, suggesting that these autoreactive B cells may be anergic. Moreover, gene array analyses of CD21–/lo B cells revealed molecules specifically expressed in these B cells and that are likely to induce their unresponsive stage. Thus, CD21–/lo B cells contain mostly autoreactive unresponsive clones, which express a specific set of molecules that may represent new biomarkers to identify anergic B cells in humans.

ORGANISM(S): Homo sapiens

PROVIDER: GSE13917 | GEO | 2009/12/01

SECONDARY ACCESSION(S): PRJNA110339

REPOSITORIES: GEO

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