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An NF-κB-responsive long noncoding RNA, PINT, regulates inflammatory gene transcription by scaffolding p65 and EZH2

ABSTRACT: Long non-coding RNAs (lncRNAs) are central regulators of the inflammatory response and play an important role in inflammatory diseases. PINT has been reported to be involved in tissue development and tumorigenesis. However, potential functions of PINT in the innate immune system are largely unknown. Here, we report a specific role for PINT in activating inflammatory gene transcription. The expression of PINT is primarily dependent on the NF-κB signaling pathway in macrophages and intestinal epithelial cells. Functionally, PINT selectively promotes the expression of inflammatory genes, including TNF-α, in basal and LPS-stimulated cells. Mechanistically, PINT acts as a modular scaffold of p65 and EZH2 to coordinate their localization and specify their binding to the target genes. Further, a high expression of PINT is detected in the intestinal tissue from patients with Inflammatory Bowel Disease (IBD). Together, these findings demonstrate that PINT acts as an activator of inflammatory responses, highlighting the importance of this lncRNA in inflammatory diseases as a potential therapeutic target.

ORGANISM(S): Homo sapiens

PROVIDER: GSE139278 | GEO | 2022/10/01

REPOSITORIES: GEO

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An NF-κB-responsive long noncoding RNA, PINT, regulates inflammatory gene transcription by scaffolding p65 and EZH2

Project description:An NF-κB-responsive long noncoding RNA, PINT, regulates inflammatory gene transcription by scaffolding p65 and EZH2

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