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Gene expression in pylorus stomach tissue in Bapx1 (Nkx3.2) deficient mice

ABSTRACT: Bapx1 (Nkx3.2) is an important factor for GI development. We document a morphologic change in the pylorus segment of the GI tract in Bapx1 deficient mice and we have used microarray analyses to assess gene exprofiles in the pylorus stomach region in knockout compared to wildtype littermates. Keywords: Genetic modiufied mice deficient in Bapx1 expression

ORGANISM(S): Mus musculus

PROVIDER: GSE13935 | GEO | 2009/02/09

SECONDARY ACCESSION(S): PRJNA110269

REPOSITORIES: GEO

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Mus musculus

Project description:Gene expression in pylorus stomach tissue in Bapx1 (Nkx3.2) deficient mice

| PRJNA110269 | ENA

Transcription profiling of mouse stomach, pylorus and duodenum tissue from E14.5 and E16.5 embryos

Project description:In the adult mouse, distinct morphological and transcriptional differences separate stomach from intestinal epithelium. Remarkably, the epithelial boundary between these two organs is literally one cell thick. This discrete junction is established suddenly and precisely at embryonic day (E) 16.5, by sharpening a previously diffuse intermediate zone. In the present study, we define the dynamic transcriptome of stomach, pylorus and intestinal tissues between E14.5 and E16.5. We show that establishment of this boundary is concomitant with the induction of over a thousand genes in intestinal epithelium, and these gene products provide intestinal character. Hence, we call this process intestinalization. We identify specific transcription factors (Hnf4g, Creb3l3 and Tcfec) and examine signaling pathways (Hedgehog and Wnt) that may play a role in this process. Finally, we define a unique expression domain at the pylorus itself and detect novel pylorus-specific patterns for the transcription factor Gata3 and the secreted protein nephrocan. Experiment Overall Design: Stomach, pylorus and duodenum tissue from E14.5 and E16.5 mouse embryos were collected for RNA extraction and hybridization on Affymetrix microarrays. We sought to study the gene expression profiles and identify genes and pathways enriched in these three tissues at two important developmental times.

2009-10-09 | E-GEOD-15872 | biostudies-arrayexpress

AQP5-Expressing Cells Serve as Stem Cells and Cancer Origins in the Mouse and Human Pyloric Stomach

Project description:Isolating adult stem cells from human stomach epithelia has proven challenging due to a lack of surface markers that are amenable to antibody-based sorting approaches. Here, through comparative expression profiling of mouse Lgr5+ adult stem cells along the GI tract, we identify novel pylorus-specific stem cell markers, including the membrane protein Aqp5. FACS-based isolation of endogenous AQP5+ cells from healthy human pyloric stomach revealed their stem cell identity in organoid assays. Using new Aqp5-driven CreERT2 mouse models to selectively target conditional mutations to the pyloric stem cell compartment in vivo, we establish pyloric stem cells as a major source of Wnt-driven invasive gastric cancer. The new stem cell markers and mouse models described here will be an invaluable resource for deciphering early gastric cancer formation and for isolating and characterizing human stomach stem cells as a prerequisite to potentially exploiting their regenerative medicine potential in the clinic.

2019-12-09 | GSE121803 | GEO

Dynamic patterning at the pylorus: formation of an epithelial intestine-stomach boundary in late fetal life

2009-10-01 | GSE15872 | GEO

Gene Expression profiles of Bapx1 sorted cells from spleen of wildtype (WT) and BApx1 null (HOMO) E12.5 mouse embryos

Project description:This study aims to look at gene expresion profiles between wildtype and Bapx1 knockout cells of the spleen in a E12.5 mouse embryo. Instead of looking at the whole spleen,only cells expressing Bapx1 were sorted by Fluroscent Activated Cell Sorting (FACS) and subjected to expression profiling by microarray.

2015-03-19 | GSE35654 | GEO

Gene Expression profiles of Bapx1 sorted cells from gut of wildtype (WT) and Bapx1 null (HOMO) E12.5 mouse embryos

Project description:This study aims to look at gene expresion profiles between wildtype and Bapx1 knockout cells of the gut in a E12.5 mouse embryo. Instead of looking at the whole gut, only cells expressing Bapx1 were sorted by Fluroscent Activated Cell Sorting (FACS) and subjected to expression profiling by microarray.

2015-03-19 | GSE35650 | GEO

Gene Expression profiles of Bapx1 sorted cells from hindlimbs (HL) of wildtype (WT) and Bapx1 null (HOMO) E12.5 mouse embryos

Project description:This study aims to look at gene expresion profiles between wildtype and Bapx1 knockout cells of the hindlimbs in a E12.5 mouse embryo. Instead of looking at the whole hindlimbs,only cells expressing Bapx1 were sorted by Fluroscent Activated Cell Sorting (FACS) and subjected to expression profiling by microarray.

2015-03-19 | GSE35652 | GEO

Gene Expression profiles of Bapx1 sorted cells from forelimbs (FL) of wildtype (WT) and Bapx1 null (HOMO) E12.5 mouse embryos

Project description:This study aims to look at gene expresion profiles between wildtype and Bapx1 knockout cells of the forelimbs in a E12.5 mouse embryo. Instead of looking at the whole forelimbs, only cells expressing Bapx1 were sorted by Fluroscent Activated Cell Sorting (FACS) and subjected to expression profiling by microarray.

2015-03-19 | GSE35649 | GEO

Gene Expression profiles of Bapx1 sorted cells from vertebral column of wildtype (WT) and Bapx1 null (HOMO) E12.5 mouse embryos

Project description:This study aims to look at gene expresion profiles between wildtype and Bapx1 knockout cells of the vertebral column in a E12.5 mouse embryo. Instead of looking at the whole vertebral column ,only cells expressing Bapx1 were sorted by Fluroscent Activated Cell Sorting (FACS) and subjected to expression profiling by microarray.

2015-03-19 | GSE35655 | GEO

In vivo and in vitro Genome wide binding (ChIP-Seq) of Bapx1 and Sox9

Project description:Bapx1 was endogenously tagged with S-Peptide and dissected vertebral columns from these tagged embryos at E12.5 were subjected to immunoprecipitation by S-peptide antibody. Simultaneously V5 tagged Bapx1 was also over expressed in NIH_3T3 cells and immunoprecipitated with V5 antibody. Concurrently vertebral column fro E12.5 mouse embryos were subjected to immunoprecipitation with Sox9 antobody to compare binding sites of Sox9 and BApx1 in the vertebral column of developing mouse embryo

2015-03-19 | GSE36672 | GEO

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