Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Alpha Ketoglutarate Prevents Age-related Osteoporosis via Regulating Histone Methylations

ABSTRACT: The aim of this study is to investigate the effect of aKG treatment on the self-renewal and lineage specification of mouse bone marrow derived mesenchymal stem/stromal cells (mBMSCs). The sequence reads were mapped to mouse genome (build mm10) using TopHat followed by Cufflinks. Expression of key genes was validated by SYBR Green RT-qPCR assays.

ORGANISM(S): Mus musculus

PROVIDER: GSE139496 | GEO | 2020/09/08

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Alpha-ketoglutarate ameliorates age-related osteoporosis via regulating histone methylations.

Project description:Age-related osteoporosis is characterized by the deterioration in bone volume and strength, partly due to the dysfunction of bone marrow mesenchymal stromal/stem cells (MSCs) during aging. Alpha-ketoglutarate (αKG) is an essential intermediate in the tricarboxylic acid (TCA) cycle. Studies have revealed that αKG extends the lifespan of worms and maintains the pluripotency of embryonic stem cells (ESCs). Here, we show that the administration of αKG increases the bone mass of aged mice, attenuates age-related bone loss, and accelerates bone regeneration of aged rodents. αKG ameliorates the senescence-associated (SA) phenotypes of bone marrow MSCs derived from aged mice, as well as promoting their proliferation, colony formation, migration, and osteogenic potential. Mechanistically, αKG decreases the accumulations of H3K9me3 and H3K27me3, and subsequently upregulates BMP signaling and Nanog expression. Collectively, our findings illuminate the role of αKG in rejuvenating MSCs and ameliorating age-related osteoporosis, with a promising therapeutic potential in age-related diseases.

| S-EPMC7645772 | biostudies-literature

Alpha Ketoglutarate Prevents Age-related Osteoporosis via Regulating Histone Methylations

Project description:Alpha Ketoglutarate Prevents Age-related Osteoporosis via Regulating Histone Methylations

| PRJNA579985 | ENA

Next Generation Sequencing measuring Nod-like receptors mRNA expression in intestinal epithelial cells

Project description:Intestinal epithelisal cells were obtained by EDTA isolation of ileum from WT mice followed by facs sorting (EpCAM + CD45 – 7AAD – ). Raw sequencing reads were aligned to the mouse genome (mm10) with Tophat , and gene expression levels were measured by Cufflinks .

2017-06-29 | GSE98388 | GEO

α-Ketoglutarate attenuates Wnt signaling and drives differentiation in colorectal cancer [RRBS]

Project description:Genetic-driven deregulation of the Wnt pathway is crucial but not sufficient for colorectal cancer (CRC) tumourigenesis. Here, we show that environmental glutamine restriction further augments Wnt signaling in APC mutant intestinal organoids to promote stemness and leads to adenocarcinoma formation in vivo via decreasing intracellular alpha-ketoglutarate (aKG) levels. aKG supplementation is sufficient to rescue low-glutamine induced stemness and Wnt hyperactivation. Mechanistically, we found that aKG promotes hypomethylation of DNA and histone H3K4me3, leading to an upregulation of differentiation-associated genes and downregulation of Wnt target genes, respectively. Using CRC patient-derived organoids and several in vivo CRC tumour models, we show that aKG supplementation suppresses Wnt signaling and promotes cellular differentiation, thereby significantly restricting tumour growth and extending survival. Together, our results reveal how the low glutamine metabolic microenvironment impacts Wnt signaling and identify aKG as a potent antineoplastic metabolite for potential differentiation therapy for CRC patients.

2020-03-23 | GSE140264 | GEO

Dietary intake of α-ketoglutarate ameliorates α-synuclein pathology in a Parkinson’s disease mouse model

Project description:In order to investigate the effect of Alpha-Ketoglutarate (AKG) on p-α-synuclein in substantia nigra of Parkinson's disease (PD) model mice (C57BL/6), we profiled substantia nigra from wild-type (WT), AAV-α-synuclein (α-Syn), AKG and α-Syn-AKG in male mice by RNA sequencing (RNA-seq).

2022-10-02 | GSE214446 | GEO

Drosophila melanogaster

Project description:Differential Gene and Transcript Expression Analysis with TopHat and Cufflinks

| PRJNA147681 | ENA

α-Ketoglutarate attenuates Wnt signaling and drives differentiation in colorectal cancer [methylation array]

2020-03-23 | GSE140265 | GEO

α-Ketoglutarate attenuates Wnt signaling and drives differentiation in colorectal cancer [exome-seq]

2020-03-23 | GSE140263 | GEO

Next-generation sequencing measuring mRNA expression in naïve T cells isolated from Mettl3 KO and WT mice

Project description:Naïve T cells were obtained by StemCell CD4+ T cell isolation kit of spleen from Mettl3 KO and WT mice followed by FACS sorting (CD4+CD25-CD45RB-Hi). Raw sequencing reads were aligned to the mouse genome (mm10) with Tophat, and gene expression levels were measured by Cufflinks.

2017-08-17 | GSE100277 | GEO

α-Ketoglutarate attenuates Wnt signaling and drives differentiation in colorectal cancer [RNA-seq I]

2020-03-23 | GSE140266 | GEO