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CgEfg1 and CgTec1 transcription factors controlling biofilm formation in Candida glabrata

ABSTRACT: This work reports the transcriptome-wide changes of C. glabrata cells (RNA-seq) upon 24h of biofilm formation on polystyerene surface, comparatively to planktonic conditions. The role of CgEfg1 and CgTec1 transcription factors is explored through the comparison of the transcriptome profiles of WT and CgEfg1 mutant cells, as well as the comparison between the profile of WT and CgTec1 mutant cells, in planktonic and 24h of biofilm conditions. mRNA profiles of WT and deletion mutant cells were generated by deep sequencing, in duplicate, using Illumina HiSeq. The sequence reads that passed quality filters were analyzed with TopHat followed by HTSeq. RNA-seq data allowed to identify genes whose expression is altered upon 24h of biofilm comparatively to planktonic conditions (1567 upregulated and 1505 downregulated). RNA-seq data allowed to identify genes regulated by the CgEfg1 (650 positively regulated and 514 negatively regulated) and CgTec1 (487 positively regulated and 595 negatively regulated) transcription factors in biofilm conditions. In all cases a log2fold change ≥1 and p value <0.01 was considered. The transcriptomics data was then used to guide further work to detail the function of each transcription factor in regulating biofilm formation.

ORGANISM(S): Nakaseomyces glabratus

PROVIDER: GSE140427 | GEO | 2021/04/30

REPOSITORIES: GEO

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CgEfg1 and CgTec1 transcription factors controlling biofilm formation in Candida glabrata

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