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Zscan4 binds nucleosomal microsatellite DNA and protects mouse two-cell embryos from DNA damage [ChIP-seq]

ABSTRACT: Genome-wide binding analysis of Zscan4 showed sequence-specific occupancy at (CA)n microsatellite repeats in 2C-like cells. Genome-wide occupancy profiling of FACT subunit SSRP1 in curaxin-treated mouse ESC demonstrated re-localization from transcription start sites to Zscan4 binding sites.

ORGANISM(S): Mus musculus

PROVIDER: GSE140619 | GEO | 2020/03/22

REPOSITORIES: GEO

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Zscan4 binds nucleosomal microsatellite DNA and protects mouse two-cell embryos from DNA damage [RNA-seq]

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Zscan4 binds nucleosomal microsatellite DNA and protects mouse two-cell embryos from DNA damage

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Zscan4 binds nucleosomal microsatellite DNA and protects mouse two-cell embryos from DNA damage.

Project description:Zinc finger protein Zscan4 is selectively expressed in mouse two-cell (2C) embryos undergoing zygotic genome activation (ZGA) and in a rare subpopulation of embryonic stem cells with 2C-like features. Here, we show that Zscan4 specifically recognizes a subset of (CA)n microsatellites, repeat sequences prone to genomic instability. Zscan4-associated microsatellite regions are characterized by low nuclease sensitivity and high histone occupancy. In vitro, Zscan4 binds nucleosomes and protects them from disassembly upon torsional strain. Furthermore, Zscan4 depletion leads to elevated DNA damage in 2C mouse embryos in a transcription-dependent manner. Together, our results identify Zscan4 as a DNA sequence-dependent microsatellite binding factor and suggest a developmentally regulated mechanism, which protects fragile genomic regions from DNA damage at a time of embryogenesis associated with high transcriptional burden and genomic stress.

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Zscan4 binds nucleosomal microsatellite DNA and protects mouse two-cell embryos from DNA damage

Project description:This SuperSeries is composed of the SubSeries listed below.

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Zscan4 binds nucleosomal microsatellite DNA and protects mouse two-cell embryos from DNA damage [ATAC-seq]

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CHIP-seq analysis about Histones and Zscan4 after Zscan4 overexpression

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