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Chemical induced senescence in the cortical neurons

ABSTRACT: The goals of this study are to compare NGS-derived transcriptome profiling (RNA-seq) of cortical neurons derived from human embryonic stem cells (H9) treated with compounds that are inducing senescence

ORGANISM(S): Homo sapiens

PROVIDER: GSE141028 | GEO | 2020/06/01

REPOSITORIES: GEO

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Chemical induced senescence in the cortical neurons

Project description:Chemical induced senescence in the cortical neurons

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Gene expression from human iPSC derived cortical neurons

Project description:Gene expression from icas9 human iPSC derived cortical neurons treated with and without doxycycline

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Cortical organoids in Huntington’s disease reveal a chaotic premature corticogenesis cascade and precede HD subcortical projections

Project description:Bulk RNA-seq characterization of human cortical organoids derived from two HD patients (CAG55 and 59) and one healthy (CAG19) sibling's induced pluripotent stemm cells (iPSCs) and human embryonic stemm cells H9 .

2023-12-11 | GSE220847 | GEO

RNA-seq-mediated transcriptome analysis of mouse primary cortical neurons

Project description:Purpose: RNA-seq analysis of the Transcript-Level change during the ferroptosis process. The goals of this study are to analyze the transcriptome profiling (RNA-seq) of the mouse cortical neurons after Hemin (50 μM) and/or 4-OI (10 μM) treatment.

2022-11-27 | GSE189652 | GEO

Sprouting transcriptome in cortical neurons

Project description:This SuperSeries is composed of the following subset Series: GSE24440: Sprouting transcriptome in cortical neurons: young GSE24441: Sprouting transcriptome in cortical neurons: aged Refer to individual Series

2010-11-15 | E-GEOD-24442 | biostudies-arrayexpress

Single cell transcriptomics analysis of induced pluripotent stem cell-derived cortical neurons reveals frequent dual layer identity

Project description:Induced pluripotent stem cell (iPSC)-derived cortical neurons present a powerful new model of neurological disease. Previous work has established that differentiation protocols produce cortical neurons but little has been done to characterise these at cellular resolution. In particular, it is unclear to what extent in vitro two-dimensional, relatively disordered culture conditions recapitulate the development of in vivo cortical layer identity. Single cell multiplex RT-qPCR was used to interrogate the expression of genes previously implicated in cortical layer or phenotypic identity in individual cells. Unexpectedly, 22.7% of neurons analysed frequently co-expressed canonical fetal deep and upper cortical layer markers, and this co-expression was also present at the level of translated protein. By comparing our results to available single cell RNA-seq data from human fetal and adult brain, we observed that this co-expression of layer markers was also seen in primary tissue. These results suggest that establishing neuronal layer identity in iPSC-derived or primary cortical neurons using canonical marker genes transcripts is unlikely to be informative. Single cell RNA-seq of 16 iPSC-derived cortical neurons. This dataset was used for normalization purposes for GSE67835.

2016-01-08 | E-GEOD-69790 | biostudies-arrayexpress

PHF6 knockdown in rat cortical neurons

Project description:E18 embryonic rat cortical neurons cultured in vitro are infected with lentivirus expressing control or PHF6shRNA-2, and harvested 5 days after infection pLL3.7 lentivirus expressing control or PHF6shRNA-2 was generated in 293T cells and concentrated using ultracentrifuge. In vitro cultured cortical neurons were infected and RNA was harvested 5 days after infection. PHF6 knockdown was validated by QPCR before sample was processed for microarray analysis.

2013-08-01 | E-GEOD-45953 | biostudies-arrayexpress

Identification of the cortical neurons that mediate antidepressant responses

Project description:This SuperSeries is composed of the following subset Series: GSE35751: Comparative analysis of S100a10-expressing cortical pyramidal cells and whole cortex GSE35758: Comparative analysis of S100a10 and Glt25d2 cortical pyramidal cells GSE35761: Effect of fluoxetine treatment on translational profiles of S100a10 cortical pyramidal cells GSE35763: Effect of fluoxetine treatment on translational profiles of Glt25d2 cortical pyramidal cells GSE35765: Effect of fluoxetine treatment on translational profiles of S100a10 cortical pyramidal cells in p11 KOs Refer to individual Series

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RNA-seq of human and mouse stem-cell derived neurons and mouse primary cortical neurons after KCl-induced membrane depolarization against controls.

Project description:This experiment comprises RNA-seq data used to study evolutionary differences between humans and mice in neuronal activity-dependent transcriptional responses. Activity-dependent transcriptional responses in developing human stem cell-derived cortical neurons were compared with those induced in developing primary- or stem cell-derived mouse cortical neurons 4 hours after KCl-induced membrane depolarisation. Activity-dependent transcriptional responses were also measured in aneuploid mouse neurons carrying human chromosome 21, allowing study of the regulation of Hsa21 genes, plus their mouse orthologs, side-by-side in the same cellular environment of a mouse primary neuron.

2017-02-16 | E-MTAB-5489 | biostudies-arrayexpress

The age and genomic integrity of neurons after cortical stroke in humans

Project description:It has been unclear whether ischemic stroke induces neurogenesis or neuronal DNA-rearrangements in the human neocortex. We show here that neither is the case, using immunohistochemistry, transcriptome-, genome- and ploidy-analyses, and determination of nuclear bomb test-derived 14C-concentration in neuronal DNA. A large proportion of cortical neurons display DNA-fragmentation and DNA-repair short time after stroke, whereas neurons at chronic stages after stroke show DNA-integrity, demonstrating the relevance of an intact genome for survival. Analyze of potential fusion transcripts in 13 samples, seven cortical ischemic stroke tissue and six control cortex, by deep sequencing using Illumina HiSeq 2000

2014-03-28 | E-GEOD-56267 | biostudies-arrayexpress

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