Methylation profiling

Dataset Information

0

The role of Vitamin C and TET dioxygenases in genome-wide features of regulatory T cells [WGBS]


ABSTRACT: TET enzymes are essential for the stability and function of regulatory T cells (Tregs), which maintain immune homeostasis and self-tolerance and express the lineage-determining transcription factor Foxp3. We previously showed that Vitamin C acts through TET enzymes to maintain the demethylated status of CNS2, a key intronic enhancer of the Foxp3 gene that is essential for the stability of Foxp3 expression both in vivo and in vitro. Here we show that Vitamin C enables genome-wide features of “induced” T regulatory cells (iTregs) in vitro that overlap with those induced by TET proteins during Treg differentiation in vivo. Vitamin C enhances IL-2 responsiveness in iTregs by increasing phospho-STAT5 levels, STAT5 occupancy and DNA demethylation at key Treg-specific enhancers, and maintains the stable expression of Treg-specific genes including Foxp3 and Il2ra. Our data will be relevant to future studies of the association between plasma Vitamin C levels, Treg function and autoimmunity in humans.

ORGANISM(S): Mus musculus

PROVIDER: GSE141151 | GEO | 2021/07/21

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2021-07-21 | GSE141150 | GEO
2021-07-21 | GSE141149 | GEO
2021-07-21 | GSE141148 | GEO
2014-09-04 | E-GEOD-57272 | biostudies-arrayexpress
2017-06-22 | GSE96960 | GEO
2014-09-04 | GSE57272 | GEO
2014-08-12 | E-GEOD-60318 | biostudies-arrayexpress
2023-11-01 | GSE246431 | GEO
2018-10-26 | GSE121764 | GEO
2014-08-12 | GSE60318 | GEO