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HSV-1 infection of neural system-derived cells, including SH-SY5Y and U87MG cells at 0, 4 and 10 hour post infection with an MOI of 0.01,to identify the differentially expressed miRNAs and perform pathway enrichment analysis using KEGG.

ABSTRACT: we employed human miRNA Microarray assay to identify differentially expressed (DE) miRNAs in response to HSV-1 infection of SH-SY5Y and U87MG cells. The significantly DE miRNAs existed in U87MG but not SH-SY5Y cells at 4 hours post-infection (hpi), HSV-1 latency-associated transcripts (LAT)-derived miRNAs were not abundantly expressed in both SH-SY5Y and U87MG cells, meanwhile, HSV-1 replication was significantly inhibited in U87MG but not SH-SY5Y. Pathway enrichment analysis results showed that target genes of 10 down-regulated DE miRNAs were significantly enriched in Janus kinase-signal transducers and activator of transcription (JAK-STAT) signaling and Herpes simplex virus infection in U87MG cells at 4 hpi. These results indicated that DE of miRNAs may contribute to the HSV-1 replication inhibition in U87MG cells and also may be linked to HSV-1 latency in neurons.

ORGANISM(S): Homo sapiens

PROVIDER: GSE141241 | GEO | 2019/12/03

REPOSITORIES: GEO

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