Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from adult mouse pancreas.

ABSTRACT: Two strains of rodent C57Bl/6J and KK/HIJ were compared to find the most suitable rodent model of obesity and type 2 diabetes. KK/HIJ become larger and glucose intolerant on standard chow but are not as well characterized as the C67Bl/6J model which has a more defined behavioral phenotype. The present study is to asses and determie the most suitable mouse model for our research. We used microarray analysis to examine changes in adult rodent pancreatic gene expression.

ORGANISM(S): Mus musculus

PROVIDER: GSE141313 | GEO | 2021/03/16

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Expression data from adult mouse adrenal tissue.

Project description:Two strains of rodent C57Bl/6J and KK/HIJ were compared to find the most suitable rodent model of obesity and type 2 diabetes. KK/HIJ become larger and glucose intolerant on standard chow but are not as well characterized as the C67Bl/6J model which has a more defined behavioral phenotype. The present study is to asses and determie the most suitable mouse model for our research. We used microarray analysis to examine differences in gene expression in C57Bl/6J and KK/HIJ , male and female adult rodent mice

2021-03-16 | GSE141310 | GEO

Dietary intervention reverses molecular signatures of hepatocellular senescence in the GAN diet-induced obese and biopsy-confirmed mouse model of NASH

Project description:We applied RNA sequencing (RNA-seq) to study the effects of dietary intervention on hallmarks of NASH and molecular signatures of hepatocellular senescence in the Gubra-Amylin NASH (GAN) diet-induced obese (DIO) mouse model of NASH. GAN DIO-NASH mice with liver biopsy-confirmed NASH and fibrosis received dietary intervention by switching to chow feeding (chow reversal) for 8, 16, or 24 weeks. Untreated GAN DIO-NASH mice and chow-fed C57BL/6J mice served as controls. We find that chow reversal promoted substantial benefits on metabolic, biochemical and histological outcomes accompanied by marked suppression of gene expression markers of hepatocellular senescence in GAN DIO-NASH mice. These therapeutic benefits were reflected by progressive clearance of senescent hepatocellular cells, making the model suitable for profiling potential senotherapeutics in preclinical drug discovery for NASH.

2024-01-16 | GSE246328 | GEO

Differential expression of genes in pancreatic islet of C57Bl/6J on high fat diet

2011-11-20 | GSE23325 | GEO

Apolipoprotein A-IV reduced metabolic inflammation in white adipose tissue by inhibiting IKK and JNK signaling in adipocytes

Project description:ApoA-IV produced by enterocytes as a major component of high-density lipoprotein plays a role in the satiation signal and also the transport of serum lipids. The present study aimed to investigate the effects of ApoA-IV on diet-induced obesity (DIO), metabolic inflammation and the underlying mechanisms. C57BL/6J ApoA-IV-/- (KO) and wildtype (WT) mice as well as KO mice stably transfected with ApoA-IV-green fluorescent protein (KO-A4-GFP) or solely GFP (KO-GFP) encoding adenoviruses were fed with standard 5% fat rodent chow (CD) or 60% high fat rodent chow (HFD) for 16 weeks.

2022-10-25 | GSE211980 | GEO

KKTa_isolated glomeruli

Project description:High dietary fat intake is a major risk factor for the development of obesity, which is frequently associated with diabetes. To identify genes involved in diabetic nephropathy, GeneChip Expression Analysis was employed to survey the glomerular gene expression profile in diabetic KK/Ta mice fed with a high-fat diet (HFD). Isolated glomeruli from three 20-week-old KK/Ta mice fed with HFD (HFD group) or a normal fat diet (Chow group) were dissected. Total RNA was extracted and labeled for hybridization using the Affymetrix GeneChip Mouse Genome 430 2.0 Array. The gene expression profile was compared between the HFD and Chow groups using GeneSpring 7.3.1 software.

2010-12-01 | E-GEOD-19251 | biostudies-arrayexpress

Differential expression of genes in pancreatic islet of C57Bl/6J on high fat diet

Project description:Male C57Bl/6J mice were fed 45%kcal fat diet (HF) or regular rodent chow (NC) from 4 weeks to 16 weeks of age. Gene expression was compared between RNA obtained from pancreatic islets of HF fed mice and NC mice. RNA samples from 4 NC group and 4 HF groups were analyzed using GeneChip Mouse Expression Arrays MOE 430v2 (Affymetrix).

2011-11-20 | E-GEOD-23325 | biostudies-arrayexpress

Fat transcriptome patterns associated with heterogeneous metabolic, hormonal and behavioral adaptation to high fat diet feeding in mice

Project description:The effect of high fat diet feeding on adipose tissue gene transcription regulation was investigated in C57Bl/6J mice using Affymetrix gene expression arrays. Expression profiling was determined in 5 months old male mice showing heterogeneous metabolic, hormonal and behavioral adaptation to high fat diet (40% fat) feeding for 15 weeks. Control mice were fed a standard carbohydrate chow. Six animals per group were used.

2014-04-13 | E-MTAB-2569 | biostudies-arrayexpress

Gene expression comparison of liver tissue from C57BL/6J and KK/HIJ mice

Project description:A QTL intercross was performed bewteen C57BL/6J and KK/HIL for albuminurea, asthma and cardiovascular related phenotypes. Several QTL were identified for most phenotypes. We performed microarray analysis from liver samples to identify genes differentially expressed between the parental strains. The results helped us narrow down the QTL and identify the candidate genes based on differential expression between the parental strains. At 8 weeks of age, the large lobe of the liver from 3 male and 3 females C57BL/6J and KK/HIJ (12 mice total) was collected. The mice were separated individuallly for 3 days and fasted for 4 hours prior to the tissue collection. The mice were sacrificed by cervically dislocation and perfused with PBS prior to the liver collection.

2012-10-18 | E-GEOD-37429 | biostudies-arrayexpress

Expression profiling of F2 progenies from C57BL/6J x DBA/2J

Project description:Expression profiling of a cohort of mice was derived from a standard F2 intercross using two standard inbred strains, C57BL/6J and DBA/2J. In this study, we selected 127 male animals that had been raised on a standard rodent chow diet (Purina Chow from Ralston-Purina Co., St. Louis, MO) for eight weeks and then switched to a high fat diet for sixteen additional weeks. At 20 weeks of age, the mice were euthanized and liver tissues were collected and flash frozen in liquid nitrogen and stored at -80 degrees C prior to RNA isolation. All procedures of housing and treatment of animals were performed in accordance with IACUC regulations.

2011-06-01 | GSE16886 | GEO

Differential expression of genes in pancreatic islets from high fat fed AKR/J and C57Bl/6J

Project description:Increased fat intake is associated with obesity and insulin resistance. In some individuals, a failure of pancreatic b-cells to increase insulin production in response to the high demands of obesity leads to diabetes. We sought to determine whether the impaired b- cell adaptation in obesity is associated with differential expression of genes involved in b-cell expansion and intermediary metabolism. Two strains of inbred mice prone to obesity, C57Bl/6J and AKR/J, were fed regular rodent chow or high-fat diet, after which islet morphology, secretory function and gene expression were assessed. AKR/J had lower blood glucose and higher insulin levels compared with C57Bl/6J mice on regular rodent chow or high fat diet. Insulin secretion was 3.2 fold higher in AKR/J than C57Bl/6J mice following intraperitoneal glucose injection. Likewise, glucose-stimulated insulin secretion from isolated islets was higher in AKR/J. Additionally, islet mass was 1.4 fold greater in AKR/J compared with C57Bl/6J. To elucidate the factors associated with the differences in insulin, we analyzed the gene expression profiles in pancreatic islets in AKR/J and C57Bl/6J mice. Of 14,000 genes examined, 220 were up-regulated and 286 were down-regulated in islets from diet-induced obese AKR/J mice compared with C57Bl/6J mice. Key genes involved in islet signaling and metabolism, e.g. glucagon like peptide-1 receptor, sterol Co-A desaturase 1 & 2 and fatty acid desaturase 2 were upregulated in obese AKR/J mice. The expression of multiple extracellular matrix proteins was also increased in AKR/J mice, suggesting a role in modulation of islet mass. Functional analyses of differentially regulated genes hold promise for elucidating factors linking obesity to alterations in islet function. Keywords: response to high fat diet

2008-02-28 | GSE10639 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data