Transcriptome analysis between primary and iPS-derived monocytes and macrophages and comparison of iPS-derived macrophages between CCR5 patients and healthy controls
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ABSTRACT: iPS-derived monocytes and macrophages are similar with primary monocytes and macrophages compared to iPS cells from the genome-wide overview and have similar gene expression patterns.
Project description:Transcriptome analysis between primary and iPS-derived monocytes and macrophages and comparison of iPS-derived macrophages between CCR5 patients and healthy controls
Project description:To study monocyte and macrophage activation in ANCA-associtated vasculitis (AAV), we performed bulk RNA sequencing of bead-selected monocytes and in vitro cultured monocyte-derived macrophages from AAV patients and healthy controls. Overview patients included for sequencing monocytes: - AAV active disease, n=4, MPO-AAV=4 - AAV remission, n=10, PR3-AAV=5, MPO-AAV=5 - Healthy controls, n=6 Overview patients included for sequencing monocyte-derived macrophages: - AAV active, n=1, PR3-AAV=1 - AAV remission, n=3, PR3-AAV=3 - Healthy controls, n=3