Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Intake of protein modulate immune function, POMC processing and IGF signaling gene expression pathways in peripheral mononuclear cells in home-dwelling old subjects - a transcriptomic approach

ABSTRACT: Adequate intake of protein among older adults is associated with better health outcomes, but conflicting results makes it difficult to define the optimal intake. To further understand the impact of protein intake on metabolic processes we studied genome-wide gene expression changes in peripheral blood mononuclear cells (PBMCs) in home-dwelling old subjects after increased protein intake for 12 weeks.

ORGANISM(S): Homo sapiens

PROVIDER: GSE141674 | GEO | 2019/12/10

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Inflammatory markers and frailty in home-dwelling elderly, a cross-sectional study

Project description:Low-grade, chronic inflammation during ageing (“inflammageing”) is suggested to be involved in the development of frailty in older age. However, studies on the association between frailty, using the frailty index definition, and inflammatory markers are limited. The aim of this study was to investigate the relationship between inflammatory markers and frailty index (FI) in older, home-dwelling adults. Home-dwelling men and women aged ≥ 70 years old, living in South-East Norway were recruited and included in a cross-sectional study. The FI used in the current study was developed according to Rockwood’s frailty index and included 38 variables, resulting in an FI score between 0 and 1 for each participant. Circulating inflammatory markers (IL-6, CRP, IGF-1, cystatin C, cathepsin S, and glycoprotein Acetyls) were analyzed from non-fasting blood samples using ELISA. Whole-genome PBMC transcriptomics was used to study the association between FI score and inflammation. The present study was a cross-sectional study that included home-dwelling men and women aged ≥ 70 years old, living in the Skedsmo area, South-East Norway. The study was conducted in 2014/2015 and has been described previously [Ottestad I, Ulven SM, Øyri LKL, Sandvei KS, Gjevestad GO, Bye A, et al. Reduced plasma concentration of branched-chain amino acids in sarcopenic older subjects: a cross-sectional study. Br J Nutr. 2018;120(4):445-53]. The participants were recruited by the National Register and received an invitation letter by mail. Briefly, a total of 2820 subjects were invited, and 437 subjects participated in the study. The participants met for a single study visit, and data was collected on dietary intake, body weight and composition, physical performance, medical history, cognitive function, risk of malnutrition, anthropometric measurements, blood pressure, heart rate, and quality of life. Non-fasting blood samples were also collected.

2023-09-01 | GSE236927 | GEO

Intake of protein modulate immune function, POMC processing and IGF signaling gene expression pathways in peripheral mononuclear cells in home-dwelling old subjects - a transcriptomic approach

Project description:Intake of protein modulate immune function, POMC processing and IGF signaling gene expression pathways in peripheral mononuclear cells in home-dwelling old subjects - a transcriptomic approach

| PRJNA594365 | ENA

Inflammatory markers and frailty in home-dwelling elderly, a cross-sectional study

Project description:Inflammatory markers and frailty in home-dwelling elderly, a cross-sectional study

| PRJNA993142 | ENA

Gut Mycobiome Dysbiosis Is Linked to Hypertriglyceridemia among Home Dwelling Elderly Danes

Project description:Gut Mycobiome Dysbiosis Is Linked to Hypertriglyceridemia among Home Dwelling Elderly Danes

| PRJEB34758 | ENA

Increased protein intake affects pro-opiomelanocortin (POMC) processing, immune function and IGF signaling in peripheral blood mononuclear cells of home-dwelling old subjects using a genome-wide gene expression approach.

Project description:BackgroundAdequate protein intake among older adults is associated with better health outcomes such as immune function and metabolic regulation of skeletal muscle, but conflicting results make it difficult to define the optimal intake. To further understand the impact of protein intake on metabolic processes, the aim of the study was to explore genome-wide gene expression changes in peripheral blood mononuclear cells (PBMCs) in home-dwelling old subjects after increased protein intake for 12 weeks.MethodIn a parallel double-blind randomized controlled intervention study, subjects (≥ 70 years) received a protein-enriched milk (2 × 20 g protein/day, n = 14, mean (±SD) age 76.9 ± 4.9 years) or an isocaloric carbohydrate drink (n = 17, mean (±SD) age 77.7 ± 4.8 years) for breakfast and evening meal for 12 weeks. PBMCs were isolated before and after the intervention. Microarray analysis was performed using Illumina technology. Serum levels of gut peptides and insulin growth factor (IGF)-1 were also measured.ResultsIn total 758 gene transcripts were regulated after increased protein intake, and 649 gene transcripts were regulated after intake of carbohydrates (p < 0.05). Forty-two of these genes were overlapping. After adjusting for multiple testing, 27 of the 758 gene transcripts were regulated (FDR, q-value < 0.25) after protein intake. Of these 25 were upregulated and two downregulated. In particular, genes and signaling pathways involved in pro-opiomelanocortin (POMC) processing, immune function, and IGF signaling were significantly altered.ConclusionsPBMCs can be used to study gene expression changes after long-term protein intake, as many signaling pathways were regulated after increased protein intake. The functional significance of these findings needs to be further investigated.Trial registrationClinicalTrials.gov, ID no. NCT02218333. The study was registered on August 18, 2014.

| S-EPMC6883584 | biostudies-literature

stone-dwelling lichen

Project description:stone-dwelling lichen Raw sequence reads

| PRJNA949288 | ENA

home dust

Project description:home dust Metagenome

| PRJNA573814 | ENA

Physical fitness in community dwelling older adults is linked to dietary intake, gut microbiota and metabolomic signatures

Project description:Physical fitness in community dwelling older adults is linked to dietary intake, gut microbiota and metabolomic signatures

| PRJEB33008 | ENA

Two transcription factors are necessary for iron homeostasis in a salt-dwelling archaeon

Project description:This SuperSeries is composed of the following subset Series: GSE29704: Two transcription factors are necessary for iron homeostasis in a salt-dwelling archaeon [gene expression data] GSE29705: Two transcription factors are necessary for iron homeostasis in a salt-dwelling archaeon [ChIP-chip data] Refer to individual Series

2011-06-02 | E-GEOD-29706 | biostudies-arrayexpress

Home composts ITS survey

Project description:Home composts ITS survey

| PRJEB9459 | ENA