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RNA-seq analysis after Ssrp1 knockout or Usp7 depletion in embryonic stem cells

ABSTRACT: Endogenous retrovirus MERVL is specifically expressed in a minority of embryonic stem cells. To determine the restrain mechanism of MERVL, we knocked out Ssrp1 and analyzed the effect on the expression of transposable elements and coding genes. Ssrp1 further interacts with ubiquitin specific protease Usp7. We knocked down usp7 and analyzed the effect on the expression of MERVL. It turns out the deletion of ssrp1 or usp7 would lead to upregulation of MERVL. This study extends our understandings of the machanism by novel factors regulates MERVL.

ORGANISM(S): Mus musculus

PROVIDER: GSE141788 | GEO | 2020/08/23

REPOSITORIES: GEO

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RNA-seq analysis about MuERVL knocked down samples

Project description:Endogenous retrovirus MERVL is specifically expressed in a minority of embryonic stem cells and 2-cell embryos. To determine the function of MERVL transcripts, we knocked down MuERVL and analyzed the effect on the expression of MERVL and its targeting genes. It turns out the downregulation of MuERVL would lead to downregulation of MERVL nearby genes. This study extends our understandings of the machanism by which MERVL regulates its neatby genes.

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