Transcriptomics

Dataset Information

0

RNA-seq experiment of shControl and shPHF8 Astrocytes


ABSTRACT: In this study we analyzed the contribution of PHF8 histone demethylase to astrocytes differentiation from mouse neural stem cells. We found that PHF8 depletion affects astocytes differentiation. Moreover, PHF8 is crucial for synaptogenesis in neurons/astrocytes cocultures. Genome wide analysis demonstrated that PHF8 controls the expression of critical astrogenic and synaptogenic genes by keeping low levels of H4K20me1 at promoters. PHF8 depletion induces aberrant astrocytes phenotype and caused a significant decrease in miniature excitatory postsynaptic currents (mEPSC) frequency and amplitude in neurons/astrocytes coclutures. These data reveal a new role of PHF8 in astrocyte differentiation and function, modulating neuronal synapse. Thus, lack of histone demethylase activity associated to PHF8 mutations might led to synapse disfunction that could directly impact into X-linked intellectual disabilities.

ORGANISM(S): Mus musculus

PROVIDER: GSE141970 | GEO | 2021/06/14

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2021-06-14 | GSE141969 | GEO
2024-02-29 | GSE257869 | GEO
2024-06-22 | PXD035635 | Pride
2024-03-11 | GSE211642 | GEO
2023-12-27 | GSE235840 | GEO
2010-04-27 | E-GEOD-20725 | biostudies-arrayexpress
2010-05-07 | E-GEOD-20563 | biostudies-arrayexpress
2017-02-14 | GSE90018 | GEO
2024-04-12 | GSE253878 | GEO
2010-04-26 | E-GEOD-20753 | biostudies-arrayexpress