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Defining effects of in utero arsenic exposure on immune cells in lungs of adult mice exposed to Influenza A with single cell RNA sequencing

ABSTRACT: Our recent studies have shown that adult mice exposed to inorganic arsenic exclusively in utero (100 ppb via maternal drinking water) exhibit greater lung damage and inflammation following infection with IAV (Influenza A virus, H1N1) than control mice not exposed in utero. We employed an unbiased single cell RNA sequencing approach to conduct a high resolution molecular analysis of recruited immune cells and their transcriptional activities in the lungs of arsenic-exposed and control mice at day 3 after IAV infection. Transcriptional profiling of single immune cells from 6 mice (3 As, 3 Con, all exposed to IAV) revealed evidence of an enhanced inflammatory response mediated by dysregulation of innate immune functions of monocyte derived macrophages, neutrophils and alveolar macrophages.

ORGANISM(S): Mus musculus

PROVIDER: GSE142047 | GEO | 2020/08/19

REPOSITORIES: GEO

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