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Embryonic erythropoiesis and hemoglobin switching require transcriptional repressor ETO2 to modulate chromatin accessibility and looping (ChIP-Seq)

ABSTRACT: To determine direct targets and the regulatory role of ETO2 in gene expression, we performed ChIPmentation with antibodies to unmodified ETO2 and ETO2 interacted factor LDB1. A de novo MEME search performed on ETO2-occupied sites, revealed enrichment for GATA and TAL binding motifs, which are the components in LDB1 complex. Nearly 86% of ETO2-binding sites were intergenic or intronic, suggesting ETO2 functions primarily in regions of the genome likely to encompass enhancers. Notabley, ETO2 occupancy was almost completely coincident with LDB1 (97% of 7,761 sites), consistent with the presence of ETO2 exclusively within the LDB1 complex and their co-function in gene regulation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE142227 | GEO | 2020/08/25

REPOSITORIES: GEO

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Embryonic erythropoiesis and hemoglobin switching require transcriptional repressor ETO2 to modulate chromatin accessibility and looping (RNA-Seq)

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Embryonic erythropoiesis and hemoglobin switching require transcriptional repressor ETO2 to modulate chromatin organization.

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Distinct Ldb1/NLI complexes orchestrate γ-globin repression and re-activation through ETO2 in human adult erythroid cells.

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2014-10-01 | E-GEOD-51485 | biostudies-arrayexpress

Human K562 cells overexpressing ETO2

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2017-05-05 | E-MTAB-4332 | biostudies-arrayexpress

Embryonic erythropoiesis and hemoglobin switching require transcriptional repressor ETO2 to modulate chromatin accessibility and looping

Project description:Embryonic erythropoiesis and hemoglobin switching require transcriptional repressor ETO2 to modulate chromatin accessibility and looping

| PRJNA596249 | ENA

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