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Gene expression profiling of side population (SP) cells and non-SP cells in Hodgkin Lymphoma (HL) cell lines_2

ABSTRACT: SP cells in HL cell lines are characterized by up-regulation of genes related to TNF-alpha Signaling via NF-κB, Inflammatory Response and Hypoxia compared to the non-SP cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE142413 | GEO | 2021/06/28

REPOSITORIES: GEO

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Gene expression profiling of side population (SP) cells and non-SP cells in Hodgkin Lymphoma (HL) cell lines_1

Project description:SP cells in HL cell lines are characterized by up-regulation of genes related to TNF-alpha Signaling via NF-κB, Inflammatory Response and Hypoxia compared to the non-SP cells.

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Gene expression profiling of side population (SP) cells and non-SP cells in Hodgkin Lymphoma (HL) cell lines

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Expression data from HTR-8/Svneo side population (SP) and non-side population (NSP) cells

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Gene expression profiling of side population (SP) cells and non-SP cells in Hodgkin Lymphoma (HL) cell lines_1

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Rabbit limbal epithelial side population (SP) cells and non-SP cells

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Expression data from HTR-8/Svneo side population (SP) and non-side population (NSP) cells

Project description:Immature cell populations, including stem cells and progenitor cells, can be found in M-bM-^@M-^\side-population (SP)M-bM-^@M-^] cells. Although SP cells isolated from some adult tissues have been reported elsewhere, isolation and characterization of human trophoblast SP cells remained to be reported. We used microarrays to detail the global program of gene expression underlying cell differentiation and identified up-regulated genes of SP HTR-8/SVneo SP cells or NSP cells were isolated using a FACS Vantage fluorescence-activated cell sorter (BD Bioscience, San Jose, California) using dual wavelength analysis (blue, 424 - 444nm; red 675 nm) after excitation with 350 nm UV light. PI-positive dead cells were excluded from the analysis. Sorted SP and NSP cells' RNA had been analyzed by microarray analysis.

2014-09-18 | E-GEOD-61547 | biostudies-arrayexpress

Expression data from HTR-8/Svneo side population (SP) and non-side population (NSP) cells

Project description:Immature cell populations, including stem cells and progenitor cells, can be found in “side-population (SP)” cells. Although SP cells isolated from some adult tissues have been reported elsewhere, isolation and characterization of human trophoblast SP cells remained to be reported. We used microarrays to detail the global program of gene expression underlying cell differentiation and identified up-regulated genes of SP cells. HTR-8/SVneo SP cells or NSP cells were isolated using a cell sorter for RNA extraction and hybridization on Affymetrix microarrays. To obtain many SP or NSP cells, we cultured a large amount of HTR-8/SVneo cells (1×10^7 cells). The cells were labeled with 2.5µg/ml Hoechst 33342 dye (Molecular Probes, Eugene, OR) for 120 minutes at 37 ℃, either alone or in combination with 50 µmol/L verapamil (Sigma-Aldrich, ST Louis, MO). Finally, the cells were then passed through EPICS ALTRA HyPerSort (Beckman Coulter, Fullerton, CA) using dual wavelength analysis (blue, 424-444 nm; red, 675 nm) after excitation with 350 nm UV light.

2011-03-12 | E-GEOD-27909 | biostudies-arrayexpress

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