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Dynamic brain cell changes after middle cerebral artery occlusion revealed by massive single cell RNAseq

ABSTRACT: We used 10XGenomics Single Cell 3' Droplet-based single cell approach to profile adult mouse cortical brain cells after middle cerebral artery occlusion.

ORGANISM(S): Mus musculus

PROVIDER: GSE142445 | GEO | 2022/12/31

REPOSITORIES: GEO

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Project description:To explore the therapeutic mechanism, we carried out an Dengzhanxixin injection experiment with a focal brain ischemic deficit rat model and explore the target proteins using TMT-based proteomics. Method are shown as follow: 1. Animals and Grouping: The animal experiments were conducted under the direction of NIH Guidelines for the Care and Use of Laboratory Animals (NIH Publications No. 80-23, revised 1996). The procedures were approved by the Animal Care and Use Committees of Beijing Normal University, China. Adult male Sprague–Dawley rats were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. The animals were allowed to acclimate for 7 d before the experiments. Rats were randomly divided into a sham-operated (sham) group, model group, Dengzhan-treated (Dengzhanxixin injection) group, and EDV-treated (edaravone, EDV) group. Dengzhan (0.36 mL/100 g) or EDV (0.18 mL/100 g) was intravenously injected into rats, the first drug treatments were performed immediately after the middle cerebral artery occlusion (MCAO) surgery, the subsequent ones were continually performed with the interval of 12 h, i.e. twice a day; and the drug treatments lasted for 7 d. The rats in the sham group and the model group were treated with the same volume of saline in the same way. 2. MCAO Surgery: Before surgery, rats were fasted overnight, with free access to water. Animals were anaesthetized with 3.5% chloral hydrate (350 mg/kg body weight, i.p.) and MCAO was performed. Briefly, the right common carotid artery, external carotid and internal carotid were exposed through a midline cut. A nylon filament with a heated rounded tip was inserted into the external carotid artery, and was pulled to the internal carotid artery to occlude the origin of the middle cerebral artery. Model animals were subject to 1.5 h of MCAO, and then to reperfusion achieved by a gentle withdrawal of the occluding thread. Sham-operated animals were subjected to the same procedure, except for the insertion of the filament.

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Dynamic brain cell changes after middle cerebral artery occlusion revealed by massive single cell RNAseq

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