ABSTRACT: Transcriptional profiling of human embryo kidney cells comparing control HEK293 transfected with empty vectors cells with HEK293 cells transfected with pcDNA3-ZNF191 (or ZNF191 siRNA vector). We searched for early ZNF191 target genes by using a transient overexpression and knockdown strategy in the human embryo kidney (HEK293) cells. A table of gene targets of transcription factor ZNF191 commonly identified by both strategies is appended below as a supplementary file.
Project description:Acute myeloid leukemia study. Supplementary Table 1: Clinical, morphological, cytogenetic and molecular genetic information on 116 AML patient samples. Supplementary Table 2: Summary of the distribution of clinical and molecular genetic characteristics within the AML sample set. Supplementary Table 3: Fluorescence ratios of the 6,283 well-measured and variably-expressed genes. Supplementary Table 4: Clinical and laboratory characteristics of normal karyotype predominant subtypes I and II. Supplementary Table 5: Supervised analysis of group-specific gene expression signatures. Supplementary Table 6: Gene-expression outcome class predictor. Supplementary Table 7: Multivariate proportional hazards analysis. Keywords: other
Project description:Total RNA samples from three biological replicates in which TFEB was transiently overexpressed in HeLa cells by transfection using a pcDNA3 vector. As negative control, we used total RNA samples from HeLa cells transfected with an empty pcDNA3 vector.
Project description:Nephronophthisis (NPHP) is a group of progressive renal disorders characterized by a variable number of cysts associated with cortical tubular atrophy. Recently, mutations in INV have been identified to be responsible for NPHP2. The inv/inv,inv-deltaC::GFP mouse that was created by introduction of inv gene lacking the C-terminus (inv-deltaC) into inv/inv mice develops multiple renal cysts without situs abnormality. Renal cyst progression was dependent on expression level of inv-deltaC transgene, suggesting that inv protein functions in a dose-dependent manner. Cell proliferation and apoptosis were increased in the inv-deltaC cystic kidneys. We searched up- and down-regulated genes during cyst development and progression using cDNA microarray. See appended table for comparison analysis of inv/inv,inv-deltaC::GFP vs +/+,inv-deltaC::GFP (GSM100334 vs GSM100335). Keywords: apoptosis, cell proliferation, gene expression, inv, inversin, polycystic kidney disease
Project description:Investigation of whole genome gene expression level changes in SH-SY5Y cells transfected pCDNA3-Pea3, pCDNA3-Erm, and pCDNA3-Er81 cells compared to pCDNA3 transfected cells as a control.
Project description:Investigation of whole genome gene expression level changes in mHypoA-2/12 cells transfected Pea3-pCDNA3, Erm-pCDNA3, and Er81-pCDNA3 cells compared to pCDNA3 transfected cells as a control.
Project description:The Supplementary files (appended below) contain the mapping for the decoding of blinded samples. This SuperSeries is composed of the SubSeries listed below.
Project description:pcDNA3-HAND1-his was transfected into HEK293T cells and harvested after 48 hours. IMAC was carried out under denaturing conditions to enrich for HAND1,and samples were loaded onto SDS-PAGE gel with HAND1 gel band excised and submitted to LC-MS/MS.
Project description:Total RNA samples from three biological replicates in which TFEB was transiently overexpressed in HeLa cells by transfection using a pcDNA3 vector. As negative control, we used total RNA samples from HeLa cells transfected with an empty pcDNA3 vector. TFEB transfection
Project description:Investigation of whole genome expression in SH-SY5Y cells transfected Elk-1-VP16 cells compared to pCDNA3 transfected cells as a control.