Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

High-throughput single-cell mitochondrial DNA genotyping reveals properties of clonal variation in human contexts

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE142745 | GEO | 2020/07/13

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

High-throughput single-cell mitochondrial DNA genotyping reveals properties of clonal variation in human contexts [CRC_scATACseq]

Project description:Natural mitochondrial DNA (mtDNA) sequence variation plays a fundamental role in human disease and enables the clonal tracing of native human cells. While various genotyping approaches revealed mutational heterogeneity in human tissues and single cells, current methodologies are limited by scale. Here, we introduce a high-throughput, droplet-based mitochondrial single-cell Assay for Transposase Accessible Chromatin with sequencing (mtscATAC-seq) protocol and computational framework that facilitate high-confidence mtDNA mutation calling in thousands of single cells. Further, the concomitant high-quality accessible chromatin readout enables the paired inference of individual cell mtDNA heteroplasmy, clonal lineage, cell state, and accessible chromatin regulatory features. Our multi-omic analyses reveals single-cell variation in heteroplasmy of a pathologic mtDNA variant (m.8344A>G), which we tie to intra-individual chromatin variability and clonal evolution. Further, using somatic mtDNA mutations, we clonally trace thousands of hematopoietic cells in vitro and in patients with chronic lymphocytic leukemia, linking epigenomic variability to subclonal evolution in vivo.

2020-07-13 | GSE148509 | GEO

High-throughput single-cell mitochondrial DNA genotyping reveals properties of clonal variation in human contexts [HumanMix]

2020-07-13 | GSE142740 | GEO

High-throughput single-cell mitochondrial DNA genotyping reveals properties of clonal variation in human contexts [CLL-scRNA]

2020-07-13 | GSE142744 | GEO

High-throughput single-cell mitochondrial DNA genotyping reveals properties of clonal variation in human contexts [CLL-scATAC]

2020-07-13 | GSE142743 | GEO

High-throughput single-cell mitochondrial DNA genotyping reveals properties of clonal variation in human contexts [CD34-invitro]

2020-07-13 | GSE142742 | GEO

High-throughput single-cell mitochondrial DNA genotyping reveals properties of clonal variation in human contexts [MERRF-C1]

2020-07-13 | GSE142741 | GEO

High-throughput single-cell mitochondrial DNA genotyping reveals properties of clonal variation in human contexts [CD34-PBMC-invivo-scATAC]

2020-07-13 | GSE148508 | GEO

High-throughput single-cell mitochondrial DNA genotyping reveals properties of clonal variation in human contexts

Project description:High-throughput single-cell mitochondrial DNA genotyping reveals properties of clonal variation in human contexts

| PRJNA598170 | ENA

High-throughput single-cell mitochondrial DNA genotyping reveals properties of clonal variation in human contexts [HumanMix]

Project description:High-throughput single-cell mitochondrial DNA genotyping reveals properties of clonal variation in human contexts [HumanMix]

| PRJNA598180 | ENA

High-throughput single-cell mitochondrial DNA genotyping reveals properties of clonal variation in human contexts [CRC_scATACseq]

Project description:High-throughput single-cell mitochondrial DNA genotyping reveals properties of clonal variation in human contexts [CRC_scATACseq]

| PRJNA624786 | ENA

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data