Transcriptomics

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Transcriptome Analysis Reveals the Topography of Local Changes after Separate Transformations of the Same Reporter in Hundreds Loci of Yeast


ABSTRACT: "Position effect" means that the activity of an integrated reporter gene (IR) depends on its genomic location. Although the impact of local environment on IR (cis-effect of location) has been widely reported, the impact of IR integration on local environment and its potential mechanism (reaction of location), such as the change of expression landscape and its fitness are poorly understood. Here, we randomly selected ~250 previously constructed strains with GFP gene inserted into hundreds of different locations separately in yeast, and performed high-throughput transcriptome sequencing.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE142839 | GEO | 2020/02/21

REPOSITORIES: GEO

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