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Identification and validation of transcription factors and open chromatin regions defining the early postnatal striatal striosome

ABSTRACT: The basal ganglia, best known for processing information required for initiation of movement, is also part of a network which regulates other aspects of movement, reward, and cognition. The major output nucleus of the basal ganglia is the striatum, and its functions are dependent on neuronal compartmentation, including into striosomes and matrix. Striatal projection neurons are GABAergic medium spiny neurons (MSNs), all of which share basic molecular signatures but are subtyped by selective expression of receptors, neuropeptides, and other gene families. Neurogenesis of the striosome and matrix occurs in separate waves, but the factors regulating terminal neuronal differentiation following migration are largely unidentified. We performed RNA- and ATAC-seq on sorted murine striosome and matrix cells at postnatal day 3 and with a focus on the striosomal compartment, validated the localization and/or role of transcription factors and their regulator(s), previously not known to be associated with striatal development, including Irx1, Foxf2, Olig2 and Stat1. In addition, we validated the enhancer function of a striosome-specific open chromatin region located 15Kb downstream of the Olig2 gene. These data and the data bases provide novel tools to dissect the networks regulating MSN compartmentation and differentiation and thus pathways relevant to movement and cognition.

ORGANISM(S): Mus musculus

PROVIDER: GSE143276 | GEO | 2021/09/26

REPOSITORIES: GEO

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