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DNAseq analysis of a genome-wide loss-of-function CRISPR/Cas9 library in haploid human embryonic stem cells (hESCs) differentiated into three germ layers

ABSTRACT: Pluripotent stem cells can differentiate into all embryonic germ layers, a process that is orchestrated by the dissolution of pluripotency network and activation of pro-differentiation pathways. Yet, the genes essential for these cell fate transitions in human are still elusive. Here, we used a genome-wide loss-of-function library established in haploid hPSCs and differentiated these cells into into the three germ layers. We performed a genetic screening of the differentited library and identified genes that are essential for the differentiation of human pluripotent stem cells (hPSCs) towards ectoderm, mesoderm and endoderm.

ORGANISM(S): Homo sapiens

PROVIDER: GSE143369 | GEO | 2020/07/30

REPOSITORIES: GEO

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