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Binding of the Treslin-MTBP Complex to Specific Regions of the Human Genome Promotes the Initiation of DNA Replication

ABSTRACT: In this study, we have mapped the binding sites for the MTBP subunit of the Treslin-MTBP complex throughout the human genome by using the CUT&RUN method. Our results have indicated that MTBP associates reproducibly with several tens of thousands of sites in the genome. Many binding sites contain either G4 DNA or an AP-1 motif in a nucleosome-free region at transcription start sites (TSSs) or enhancers, respectively. In addition, this nucleosome-free region is adjacent to a nucleosome containing certain histone marks characteristic of open chromatin that have also been implicated in initiation. We also mapped early initiation zones with EdU-labeling (EdU-seq) and found that these initiation domains contain a large number of MTBP binding sites. Taken together, these findings indicate that Treslin-MTBP associates with multiple genomic signals to promote initiation of replication.

ORGANISM(S): Homo sapiens

PROVIDER: GSE143481 | GEO | 2020/09/22

REPOSITORIES: GEO

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